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为选育抗疱疹病毒(Cy HV-3)的新品种德国镜鲤,本研究在F_1抗病家系基础上构建10个家系分A、B两组混养。从193对引物中分别筛选3对高多态性微卫星引物进行亲子鉴定及遗传结构分析。结果显示:(1)两组中3个位点的累积排除率为99.99%、99.98%,亲子代配对率为100%(p=0.05),说明利用3个高多态性位点就可达到亲子鉴定的目的。(2)家系鉴定成活率为0%~60.21%。其中1#、8#、9#、10#家系比对照组提高43.51%、22.00%、11.64%、8.45%,可用于F_3选育。(3)1#、8#、9#、10#4个家系的遗传多样性指标为:Ae为3.507 7~4.033 2、Ho为0.818 1~0.965 8、He为0.7062~0.7386,遗传多样性水平较高。(4)除9#家系的HLJ3939位点显著偏离Hardy-Weinberg平衡(p<0.05)外,其他位点均极显著偏离Hardy-Weinberg平衡(p<0.01),说明所选位点均经过了选择作用。(5)1#、8#、9#、10#4个家系FST为0.057 3~0.115 6,8#、9#、10#3个家系间的Nei氏遗传距离均值为0.515 0,说明4个家系具有较高遗传潜力。因此,本研究成功筛选出4个成活率较高且具有较高遗传潜力的家系,以用于下一代选育。
In this study, 10 new families of A and B polyculture were constructed on the basis of F 1 resistant pedigree. Three pairs of high polymorphic microsatellite primers were screened from 193 pairs of primers for paternity analysis and genetic structure analysis. The results showed that: (1) The cumulative exclusion rates of three sites in two groups were 99.99% and 99.98%, respectively, with paternity pairing rate of 100% (p = 0.05), indicating that the three high polymorphic sites The purpose of paternity test. (2) The pedigree identification survival rate was 0% ~ 60.21%. The families of # 1, # 8, # 9 and # 10 increased by 43.51%, 22.00%, 11.64% and 8.45% compared with the control group, which could be used for F_3 breeding. (3) The genetic diversity index of 4 pedigrees in 1 #, 8 #, 9 # and 10 # is: Ae is 3.507 7 ~ 4.033 2, Ho is 0.818 1 ~ 0.965 8, He is 0.7062 ~ 0.7386, genetic diversity Higher. (4) The loci of HLJ3939 deviated significantly from Hardy-Weinberg equilibrium (p <0.01) except Hardy-Weinberg equilibrium (p <0.05) in 9 # family, indicating that all the selected loci had been selected effect. (5) The average Nei’s genetic distance among 4 families of # 1, # 8, # 9 and # 10 was 0.057 3 ~ 0.115 6,8 #, 9 # and 10 # Family has high genetic potential. Therefore, this study successfully screened four families with higher survival and high genetic potential, for the next generation of breeding.