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目的:探讨HOXA远端转录本(HOXA transcript at the distal tip,HOTTIP)在食管鳞癌(esophageal squamous cell cancer,ESCC)组织中的表达及对食管癌细胞部分生物学行为的影响。方法:采用逆转录-实时定量PCR法(RT-q PCR)检测ESCC组织及细胞中HOTTIP的表达水平;通过RNA干扰技术将si-HOTTIP转入细胞中以降低HOTTIP的表达水平,并通过定量PCR检测其干扰效率。用CCK8法和Transwell法检测敲除HOTTIP对细胞增殖能力和侵袭能力的影响。结果:与癌旁组织相比,ESCC组织中HOTTIP的表达明显升高;与正常食管上皮细胞相比,ESCC细胞中HOTTIP的表达明显升高。此外,通过分析临床数据发现高表达的HOTTIP与肿瘤体积和淋巴结转移紧密相关。CCK8和transwell实验显示,在敲除HOTTIP后,Eca-109和TE-13细胞的增殖和侵袭能力明显下降。结论:HOTTIP在ESCC细胞的增殖和侵袭过程中具有相当重要的作用。
Objective: To investigate the expression of HOXA transcript at the distal tip (HOTTIP) in esophageal squamous cell carcinoma (ESCC) tissues and its effect on the biological behavior of esophageal cancer cells. Methods: The expression of HOTTIP in ESCC tissues and cells was detected by reverse transcription-real-time PCR (RT-qPCR). Si-HOTTIP was transfected into cells by RNA interference to decrease the expression of HOTTIP. Detect its interference efficiency. The effect of knockout HOTTIP on cell proliferation and invasion was examined by CCK8 assay and Transwell assay. Results: The expression of HOTTIP in ESCC tissues was significantly higher than that in adjacent non-cancerous tissues. The expression of HOTTIP in ESCC cells was significantly higher than that in normal esophageal epithelial cells. In addition, high-expression HOTTIP was found to be closely related to tumor volume and lymph node metastasis by analyzing clinical data. CCK8 and transwell experiments showed that after the removal of HOTTIP, Eca-109 and TE-13 cells significantly decreased the ability of proliferation and invasion. Conclusion: HOTTIP plays a very important role in the proliferation and invasion of ESCC cells.