论文部分内容阅读
目的探索海马内胆囊收缩素(CCK)阳性(CCK+)神经元的电生理学特性。方法用整合有CCK启动子-绿色荧光蛋白(GFP)的腺病毒对培养海马CCK+神经元进行标记,应用全细胞膜片钳技术对标记CCK+神经元的电生理学特性进行研究。结果 CCK+神经元的胞体长径为(22.85±0.77)μm,短径为(16.21±0.42)μm(n=20),静息电位(RMP)为(-55.90±1.30)mV,膜电容(Cm)为(45.77±2.06)pF,膜阻抗(Rm)为(711.00±46.69)MΩ;CCK+神经元500 ms内100 pA去极化电流诱发动作电位的发生频率为(26.17±3.41)Hz(n=12);CCK+神经元的自发抑制性突触后电流的发生频率[(5.26±0.71)Hz,n=7]显著高于自发兴奋性突触后电流的发生频率[(3.24±0.62)Hz,n=6]。结论 CCK+神经元的相关电生理学特征可能与其在中枢神经系统内重要的调节功能相关。
Objective To investigate the electrophysiological characteristics of cholecystokinin (CCK) -positive (CCK +) neurons in the hippocampus. Methods The CCK + neurons in hippocampus were labeled with adenovirus integrated with CCK promoter and green fluorescent protein (GFP), and the electrophysiological characteristics of CCK + neurons labeled with whole-cell patch clamp technique were studied. Results The long axis of CCK + neurons was (22.85 ± 0.77) μm, the short axis was (16.21 ± 0.42) μm (n = 20) and the resting potential was (-55.90 ± 1.30) mV. The membrane capacitance ) Was (45.77 ± 2.06) pF and the membrane impedance (Rm) was (711.00 ± 46.69) MΩ. The frequency of action potentials induced by 100 pA depolarization current in CCK + neurons within 500 ms was (26.17 ± 3.41) Hz 12). The frequency of spontaneous inhibitory postsynaptic currents [(5.26 ± 0.71) Hz, n = 7] in CCK + neurons was significantly higher than that of spontaneous excitatory postsynaptic currents [(3.24 ± 0.62) Hz, n = 6]. Conclusion The relevant electrophysiological characteristics of CCK + neurons may be related to their important regulatory function in central nervous system.