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目的用膜亲和层析法（ＭＡＣ）纯化小鼠腹水中单克隆抗体（ｍＡｂ）。方法采用尼龙滤膜ＺＢＭ作为介质吸附抗人血清白蛋白（ＨＳＡ），将含ｍＡｂ的腹水滤过此ＺＢＭ，再将吸附于ＺＢＭ上的ｍＡｂ解离下来，获得纯化的ｍＡｂ。结果直径５０ ｍｍ吸附了 ＨＳＡ的 ＺＢＭ，经 １０ ｍＬ腹水循环滤过两次，即可达到最大ｍＡｂ结合量。从ＺＢＭ上解离ｍＡｂ，仅需解离液滤过１次就可完成。纯化的ｍＡｈ经ＰＡＧＥ检测，只显示１条带。用纯化的ｍＡｂ做金标记斑点免疫渗滤法（ＤＩＧＦＡ）检测ＨＳＡ标准品时，其灵敏度较用未纯化的腹水时提高了２０倍。结论用尼龙滤膜ＺＢＭ改进的膜亲和层析法，可用于纯化腹水中的单克隆抗体，且简便、省时、有效。
Objective To purify murine ascites monoclonal antibody (mAb) by membrane affinity chromatography (MAC). Methods Antibodies against human serum albumin (HSA) were adsorbed on nylon membrane ZBM as a medium, ascites containing mAb were filtered through this ZBM, and then the mAb adsorbed on ZBM was dissociated to obtain purified mAb. Results The ZBM with 50 mm diameter adsorbed HSA was filtered twice by 10 mL ascites to reach the maximum binding capacity of mAb. Dissociation of the mAb from the ZBM can be done only once with the dissociation fluid filtration. Purified mAh was detected by PAGE and showed only 1 band. When using the purified mAb to do gold-labeled dot immunogold filtration assay (DIGFA), the sensitivity of HSA was 20 times higher than that of unpurified ascites. Conclusion The modified membrane affinity chromatography with nylon membrane ZBM can be used to purify the monoclonal antibody in ascites, which is simple, time-saving and effective.