以斯卑尔脱小麦的一个变种及其近等基因系为材料 ,玻璃针法进行显微操作在减数分裂中期I分裂相中获得G型细胞质雄性不育的育性恢复基因Rf3所在的 1B染色体DNA。LA -PCR方法扩增DNA ,斑点杂交的方法检测证明扩增产物来源于斯卑尔脱小麦基因组的 1B染色体DNA A Variety of Espirole Wheat and Its Near-isogenic Lines as Materials, Microscopic Operation with Glass Acupuncture Method to Obtain G-type Cytoplasmic Male Sterility Fertility Restorer Gene Rf3 in Mid-meiotic Phase I Split Phase 1B Chromosomal DNA. LA-PCR method to amplify DNA, dot blot hybridization method to verify the amplification products derived from Spelled wheat genome 1B chromosome DNA