背景:辐射损伤可使机体体内产生大量的活性氧自由基,从而对机体各部位造成过氧化损伤。葡萄籽原花青素(GSP)具有极强的抗氧化及清除自由基活性,但对辐射损伤的保护作用研究尚少。目的:观察GSP及其与酪蛋白联合对Co-γ射线照射损伤大鼠过氧化60损伤的保护作用。设计:以实验动物为研究对象的观察对比研究。单位:第三军医大学大坪医院营养科。材料:实验于2004-06/2004-07在第三军医大学野战外科研究所三室完成。研究对象为80只清洁级健康Wistar大鼠,雌雄各半,体质量180～220g。方法:实验设正常对照、照射对照、GSP、GSP+酪蛋白联合4组,每组20只,GSP组在常规饲料基础上添加1%的GSP,联合组添加1%的GSP和5%的酪蛋白,各组自由进食。10d后除正常对照组外,其余大鼠均接受607Gy的Co-γ全身一次性照射,照射后第7d,每组随机各取存活大鼠6只宰杀取材。主要观察指标:血清丙二醛(MDA)水平、血清超氧化物歧化酶(SOD)活力、脾脏指数以及外周血白细胞计数(WBC)。结果:受照后大鼠血清MDA显著升高,SOD活力显著下降,GSP组和联合组MDA显著低于照射对照组(P<0.01),SOD活力显著高于照射对照组(P<0.01)。辐射损伤后,大鼠脾脏明显萎缩,脾脏指数下降,WBC严重降低。GSP组和联合组脾脏指数明显高于照射对照组(P<0.05),WBC显著高于照射 Background: Radiation damage can produce a large number of reactive oxygen species in the body and thus cause peroxidation damage to various parts of the body. Grape seed proanthocyanidins (GSP) have strong antioxidant and scavenging free radical activity, but little is known about the protective effect of radiation damage. Objective: To observe the protective effect of GSP combined with casein on the injury of peroxisome 60 induced by Co-γ radiation in rats. Design: A comparative study of experimental animals. Unit: Third Military Medical University Daping Hospital Nutrition. Materials: The experiment was performed in three rooms of the Institute of Field Surgery, Third Military Medical University from June 2004 to July 2004. The subjects were 80 clean Wistar rats, male and female, weight 180 ~ 220g. Methods: In the experiment, normal control, irradiation control, GSP, GSP + casein were combined with 4 groups with 20 mice in each group. GSP supplemented with 1% GSP on the basis of conventional diet, 1% GSP and 5% casein All groups were free to eat. After 10 days, except for the normal control group, the other rats received 607Gy Co-γ whole body irradiation at one time. On the 7th day after irradiation, 6 rats in each group were randomly selected and killed. MAIN OUTCOME MEASURES: Serum malondialdehyde (MDA) level, serum superoxide dismutase (SOD) activity, spleen index and peripheral blood leukocyte count (WBC). Results: After irradiation, serum MDA significantly increased and SOD activity decreased significantly. The MDA level in GSP group and combined group was significantly lower than that in irradiated group (P <0.01). SOD activity was significantly higher than that in irradiated control group (P <0.01). After radiation injury, the spleen of rats significantly atrophy, spleen index decreased, WBC decreased significantly. The spleen index of GSP group and combined group was significantly higher than that of irradiated control group (P <0.05), WBC was significantly higher than that of irradiated