CsA是具有生物活性的真菌代谢产物。它有明显的免疫抑制作用,特别对T细胞有特殊的抑制作用而不影响造血。它能跨过组织配型障碍而建立移植耐受性,它预防GVHD有效,但治疗效果差,它的作用机制目前尚不清楚。实验曾表明,它对T细胞增生的抑制与其剂量有关,主要抑制溶细胞性淋巴细胞的诱导而不影响抑制性细胞的形成。本实验是体外观察Cs A诱导耐受性的机制。 CsA is a biologically active fungal metabolite. It has obvious immunosuppressive effects, especially for T cells have a special inhibitory effect without affecting hematopoiesis. It can build tolerance to transplants across tissue dysfunctions. It prevents GVHD effectively, but its efficacy is poor and its mechanism of action is unclear. Experiments have shown that its inhibition of T cell proliferation is related to its dosage, mainly inhibiting the induction of cytolytic lymphocytes without affecting the formation of inhibitory cells. This experiment is to observe the mechanism of CsA induced tolerance in vitro.