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目的:利用高糖高脂饮食加链脲佐菌素诱导的2型糖尿病小鼠对金花茶叶的乙酸乙酯/二氯甲烷提取物、正丁醇提取物、粗提物进行降糖活性评价。方法:采用高糖高脂饲料饲养加腹腔注射链脲佐菌素诱导C57BL/6J小鼠2型糖尿病,造模成功后灌胃给予小鼠金花茶叶不同的溶剂提取物(粗提物,乙酸乙酯/二氯甲烷提取物,正丁醇提取物),1次/d,连续5周,单次给药剂量为100mg/kg;以二甲双胍作为阳性参照药物,单次给药剂量为250mg/kg,实验期间观察小鼠体征、体重、饮水和饮食,给药后第1周、第4周检测空腹血糖,第5周检测口服葡萄糖耐量,实验结果表明乙酸乙酯/二氯甲烷提取物降糖效果最好;用相同的造模方法建立另一批模型小鼠用于乙酸乙酯/二氯甲烷提取物的量效关系研究:设置高(200mg/kg)、中(100mg/kg)、低(50mg/kg)三个剂量组,二甲双胍为阳性参照药物(剂量250mg/kg),于给药后第4周、第5周检测空腹血糖,第5周检测口服葡萄糖耐量。结果:(1)和模型组相比,金花茶叶的三种提取物均能不同程度地改善小鼠的体征,减少饮食和饮水;(2)和模型组相比,乙酸乙酯/二氯甲烷提取物在给药后1周可显著降低小鼠空腹血糖(P<0.01),粗提物长期给药也有明显的降糖作用;(3)三个金花茶叶提取物对小鼠口服葡萄糖耐量均没有明显的改善作用;(4)金花茶叶乙酸乙酯/二氯甲烷提取物在给药剂量50~200mg/kg范围内降糖效果与给药剂量无关。结论:金花茶叶乙酸乙酯/二氯甲烷提取物、粗提物、正丁醇提取物对小鼠2型糖尿病均有一定的改善作用,但对口服葡萄糖耐量均无明显改善作用,乙酸乙酯/二氯甲烷提取物降糖效果最佳,给药剂量在50~200mg/kg时乙酸乙酯/二氯甲烷提取物降糖效果与给药剂量无关。
OBJECTIVE: To evaluate the hypoglycemic activity of ethyl acetate/methylene chloride extract, n-butanol extract and crude extract of Jinhua tea in mice with type 2 diabetes induced by high-sugar high-fat diet plus streptozotocin . METHODS: Type 2 diabetes mellitus in C57BL/6J mice was induced by high-sugar and high-fat diet fed with intraperitoneal injection of streptozotocin. After the model was successfully established, mice were given different solvent extracts of golden flower tea (crude extract, acetic acid Ethyl ester/dichloromethane extract, n-butanol extract), once a day for 5 weeks, a single dose of 100 mg/kg; metformin as a positive reference drug, a single dose of 250 mg/ Kg, Observe mouse signs, body weight, drinking water, and diet during the experiment, test fasting blood glucose at the 1st and 4th week after administration, and test oral glucose tolerance at the 5th week. The results showed that the ethyl acetate/dichloromethane extract decreased. The sugar effect was the best; the same modelling method was used to establish a dose-effect relationship study of another batch of model mice for ethyl acetate/dichloromethane extracts: setting high (200 mg/kg), medium (100 mg/kg), In the low (50 mg/kg) three-dose group, metformin was a positive reference drug (dose 250 mg/kg), fasting blood glucose was measured at the 4th and 5th week after the administration, and oral glucose tolerance was measured at the 5th week. Results: (1)Compared with the model group, the three extracts of Jinhua tea all improved mice’s signs to varying degrees, reducing diet and drinking water; (2) Compared with the model group, ethyl acetate/dichlorobenzene Methane extracts significantly reduced fasting blood glucose in mice at 1 week after administration (P<0.01), and long-term administration of crude extracts also had significant hypoglycemic effect; (3) Three Golden Tea Extracts oral glucose in mice There was no significant improvement in tolerance; (4) The hypoglycemic effect of Jinhua Tea Ethyl acetate/dichloromethane extract doses in the range of 50-200 mg/kg was not related to the dose. Conclusion: Jinhua Tea Ethyl acetate/dichloromethane extract, crude extract and n-butanol extract all have a certain effect on the improvement of type 2 diabetes in mice, but no significant improvement in oral glucose tolerance was observed. The ester/dichloromethane extract had the best hypoglycemic effect, and the dose of ethyl acetate/dichloromethane extract was not related to the dose when the dosage was between 50 and 200 mg/kg.