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游离花生四烯酸的利用是PG生物合成的限速步骤,磷酯酶A_2(PLA_2)是催化花生四烯酸释放的关键酶。为了解妊娠早期PG合成的调节,本文研究了羊动情期和妊娠早期子宫内膜亚细胞成分的PLA_2活性。 给24只羊放置14天含300mg17α-乙酸-9-α-氟-1-β羟黄体酮阴道栓,取出栓剂后肌注500IU马促性腺激素诱导动情,分别与输精管切除或正常公羊交配。取幼情期或妊娠期12、14和16天羊子宫内膜10～65g切碎,Hanks培基冲洗,4℃三倍容积
Use of free arachidonic acid is a rate-limiting step in PG biosynthesis. Phosphatase A 2 (PLA 2) is a key enzyme that catalyzes the release of arachidonic acid. In order to understand the regulation of PG synthesis in early pregnancy, the activity of PLA 2 in endometrial subevolution during the period of estrus and early gestation was studied. 24 sheep were housed for 14 days with 300 mg 17α-acetic acid-9-α-fluoro-1-β-hydroxyprogesterone vaginal suppository. After the suppository was removed, intramuscular injection of 500 IU horse gonadotropin induced estrus, respectively, with vasectomy or normal ram mating. Shepherd or gestational 12,14 and 16 days of sheep endometrium 10 ~ 65g chopped, Hanks Pei-washing, 4 ℃ three times the volume