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本文介绍了一种改良的烧杯-高渗F_(10)游泳法,用以收集活动精子,经等渗F_(10)洗涤后行宫腔内授精以治疗男性不育症。9名不育男子(5例弱精、1例少精、3例原因不明不育症)接受治疗。为了提高活动精子回收率,采用烧杯作为精子游泳的容器,以扩大精液与F_(10)溶液间之界面及缩短精子进入溶液的游程。高渗F_(10)溶液渗透压为380mosm/1它可能利于除去精子表面的获能抑制因子。精液处理后,即得到精子密度高、活力强、正常形态率高的悬液。5例弱精症患者的活动精子百分率从29±13提高到85±12(SD)％。活动精子回收率为43.7±10.7％。在B型超声波监测下,于排卵前一天宫腔内人工授精一次。2例弱精症和1例原因不明不育症患者妻子怀孕,其中1名弱精症患者的妻子已于1986年6月生一健康女婴。作者认为,对于某些不育症,用这种改良游泳法收集精子是值得推荐的。
This paper introduces a modified beaker - hypertonic F10 swimming method to collect motile sperm. After washing with isotonic F10, intrauterine insemination is performed to treat male infertility. Nine infertile men (5 weaker, 1 less severe, 3 unexplained infertility) were treated. In order to improve the sperm recovery rate, a beaker is used as a sperm swimming container to expand the interface between the semen and the F 10 solution and shorten the run of the sperm into the solution. Hypertonic F_ (10) solution osmotic pressure of 380 mosm / 1 It may help to remove the sperm inhibitory factor of the surface. Semen processing, that is, to get sperm density, strong vitality, the normal form of high suspension. The percentage of motile sperm in 5 patients with asthenospermia increased from 29 ± 13 to 85 ± 12 (SD)%. Active sperm recovery was 43.7 ± 10.7%. In the B-mode ultrasound monitoring, intrauterine insemination one day before ovulation. Two cases of asthenospermia and one case of unexplained infertility wives were pregnant, and one of the wives of a patient with asthenospermia gave birth to a healthy baby girl in June 1986. The author believes that for some infertility, using this improved swimming method to collect sperm is recommended.