IL-6 在免疫和炎症反应中发挥多种生物学功能,其产生细胞也有多种,本文采用人全血细胞培养方法对血细胞中IL-6产生细胞类型进行了探讨。正常人血浆中IL-6活性很低(<0.1U/ml),但当用LPS 刺激全血细胞,2小时后即能在血浆中测出IL-6活性,此活性12小时达高峰(500U/ml),48小时仍保持较高水平。在全血细胞培养物中加入ConA,同样可诱导出IL-6活性,但较用LPS 刺激低一些。 IL-6 plays a variety of biological functions in immune and inflammatory reactions, and its production of cells also has a variety of IL-6-producing cell types in human blood cell culture methods. Normal plasma IL-6 activity is very low (<0.1U / ml), but when whole blood cells are stimulated with LPS, IL-6 activity is measured in plasma 2 hours later, reaching a peak at 12 hours (500 U / ml), remained high for 48 hours. The addition of ConA to whole blood cell cultures also induced IL-6 activity, but was less than stimulated with LPS.