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目的 研究硒对氟致机体损伤的保护作用及探讨其保护机理。方法 Wistar大鼠饮 3 0mg/L和 5 0mg/L高氟水造成慢性氟中毒 ,同时另外两组大鼠饮高氟水、饲 2mg/kg加硒饲料。 1年内每月测饮水摄食和排尿量 ,测尿氟含量 ;每 2月摄X线片。喂养 4、8、14个月分批处死大鼠 ,测硒氟含量、骨形态计量学参数、自由基含量、抗氧化酶类、甲状腺激素、心电图、心肌电生理参数等。结果 对氟中毒大鼠投硒可改善饮食、增加体重 ;促进尿氟排泄 ,降低体氟 ;延迟X线异常改变和氟骨症的发生同时减少骨量对骨矿化障碍产生抑制作用 ;恢复抗氧化酶类活性 ;调整甲状腺激素水平 ;拮抗氟中毒所致的心电图和心肌电生理异常。结论 一定浓度范围内的硒具有拮抗高氟作用 ,促进尿氟排泄、保护骨骼系统、调整自由基代谢及对肝、肾、心脏的作用是硒拮抗高氟的重要机制
Objective To study the protective effects of selenium on fluoride induced injury and to explore its protective mechanism. Methods Wistar rats were treated with 300 mg / L and 50 mg / L of high-fluoride water to cause chronic fluorosis. At the same time, the other two groups of rats were given fluoride water and 2 mg / kg selenium-fed diet. A monthly measurement of drinking water intake and urination volume, urine testing fluoride; every X-ray film taken in February. Rats were sacrificed 4, 8, and 14 months after seeding. Selenium content, bone metrology parameters, free radical content, antioxidant enzymes, thyroid hormones, electrocardiogram and electrophysiological parameters were measured. Results Fluorine poisoning rats selenium can improve the diet, increase body weight; promote urinary fluoride excretion, reduce body fluorine; delay X-ray abnormalities and the occurrence of skeletal fluorosis and reduce the amount of bone mineralization dysfunction; bone resisting Oxidase activity; adjust the level of thyroid hormone; antagonize fluoride poisoning caused by electrocardiogram and myocardial electrophysiological abnormalities. Conclusions Selenium at a certain concentration range has the effect of antagonizing high fluoride, promoting urinary fluoride excretion, protecting the skeletal system, regulating free radical metabolism, and acting on the liver, kidney and heart, and is an important mechanism of antagonizing high fluorine by selenium