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研究了等氮量条件下沼液与化肥配施对冬小麦根际土壤微生物(细菌、真菌和放线菌)数量及土壤酶(脲酶、蛋白酶和过氧化氢酶)活性的影响.结果表明:随着生育期的推进,不同施肥处理小麦根际土壤微生物数量和土壤脲酶、过氧化氢酶活性均呈先降后升的变化趋势,而蛋白酶活性则呈“S”形曲线变化.沼液与化肥合理配施能显著增加根际土壤微生物数量,提高土壤脲酶和蛋白酶活性,以基施50%沼液氮基础上追施50%化学氮处理和基施25%沼液氮基础上追施75%化学氮处理较好,传统尿素处理和单一沼液处理效果均较差.土壤过氧化氢酶活性以基施25%沼液氮基础上追施75%化学氮处理及单施沼液处理在所有测定时期均表现最高,其他处理在各生育时期间差异很大.沼液与尿素配合施用可以提高小麦根际土壤微生物数量和酶活性.
The effects of biogas slurry and chemical fertilizers on rhizosphere microbes (bacteria, fungi and actinomycetes) and soil enzyme activities (urease, protease and catalase) in winter wheat rhizosphere were studied under equal nitrogen conditions.The results showed that During the growth period, the amount of soil microbes and the activities of soil urease and catalase decreased first and then increased, while the protease activity showed a curve of “S” Fertilizer rational allocation can significantly increase the number of rhizosphere soil microorganisms, improve soil urease and protease activity, basing on 50% biogas slurry nitrogen topdressing 50% chemical nitrogen treatment and basal application of 25% biogas slurry nitrogen topdressing 75 % Chemical nitrogen treatment is better, the traditional urea treatment and single biogas slurry treatment effect is poor.Cat catalase activity was basal application of 25% biogas slurry nitrogen topdressing 75% chemical nitrogen treatment and single biogas slurry treatment All the determination periods showed the highest, and the other treatments differed greatly in different growth periods.The application of biogas slurry and urea could increase the amount of rhizosphere soil microbes and enzyme activity.