为了研究蕨麻中抑菌活性物质的提取工艺,以金黄色葡萄球菌和大肠杆菌为指示菌,以抑菌圈直径为活性检验指标,在单因素试验的基础上通过正交试验优化工艺参数。试验结果表明,对蕨麻抑菌活性物质提取效果的影响次序是:乙醇体积分数、提取温度、提取时间、固液比;其最佳提取工艺条件:乙醇体积分数80%,提取温度70℃,提取时间8h,固液比1:10。在此条件下,蕨麻提取液对金黄色葡萄球菌和大肠杆菌的抑菌圈直径分别为15.6mm和15.3mm。 In order to study the extraction technology of antibacterial active substances in the fern, Staphylococcus aureus and Escherichia coli were taken as the indicator bacteria, and the diameters of the bacteriostatic circles were taken as the indexes of the activity test. The parameters of the process were optimized by orthogonal test based on the single factor test. The results showed that the order of effect on the extraction of the antibacterial active substance was as follows: volume fraction of ethanol, extraction temperature, extraction time and solid-liquid ratio. The optimal extraction conditions were ethanol volume fraction 80%, extraction temperature 70 ℃, Extraction time 8h, solid-liquid ratio 1:10. Under these conditions, the inhibitory zone diameter of the extract of the fern from Staphylococcus aureus and Escherichia coli was 15.6 mm and 15.3 mm, respectively.