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用 PCR- STR、聚丙烯酰胺凝胶电泳及银染技术,对 DHFRP2、 FIBRA和 ACTBP2三个 STR位点的法科学应用进行探讨,显示三个位点的扩增效率高, DNA降解至 400 bp以下时也可扩增成功,可用于法医学个人识别。三个位点的基因型偶合率为 7.6× 10- 5,累积非父排除率达 96.81%,将此三个位点用于法科学亲子鉴定和个人识别,均获得满意结果。种属研究发现, 9种常见动物 DNA样本 (兔、鸡、鼠、牛、猪、蛙、猫、鱼和蛇 )中,兔、鸡 DNA在 DHFRP2位点有一长度为 179 bp的扩增片段;兔、牛、猪、鸡、鱼 DNA在 FIBRA位点出现一长度为 207 bp的扩增片段; 9种动物 DNA在 ACTBP2位点均无扩增产物出现。
The application of the three STR sites of DHFRP2, FIBRA and ACTBP2 by PCR-STR, polyacrylamide gel electrophoresis and silver staining revealed that the three sites had high amplification efficiency and the DNA was degraded to 400 bp The following amplification can also be successful, can be used for forensic personal identification. The genotypes coupling rate of the three loci was 7.6 × 10-5, and the accumulative non-parent exclusion rate was 96.81%. The three loci were used for scientific paternity test and personal identification, and satisfactory results were obtained. Species studies showed that the DNA of rabbits and chicken had a 179 bp fragment in DHFRP2 locus in nine common animal DNA samples (rabbit, chicken, mouse, cow, pig, frog, cat, fish and snake) Rabbit, bovine, porcine, chicken and fish DNA showed a 207 bp fragment at the FIBRA locus. None of the 9 animal DNAs showed any amplification at the ACTBP2 locus.