论文部分内容阅读
[目的]研究苯胺、硝基苯对肝癌细胞SMMC-7721的联合毒作用,为更好地预防环境化合物对人体健康损害提供理论依据。[方法]已建株的肝癌细胞SMMC-7721,分别用不同浓度的苯胺、硝基苯及其混合物(1:1,c/c)处理24h后,测定细胞存活率、细胞内活性氧(ROS)的含量及谷胱甘肽(GSH)含量、细胞的DNA链断裂情况。[结果]细胞经苯胺、硝基苯及其混合物处理24h后,其细胞存活率均随浓度的增加而降低,且混合物组的细胞存活率明显低于两种物质单独处理的细胞存活率,苯胺与硝基苯对SMMC-7721的细胞毒性可能存在协同作用。不同浓度的苯胺、硝基苯及其混合物处理24h后,与阴性对照组相比,苯胺组、苯胺+硝基苯组的彗星细胞率和彗星尾长明显增加(P﹤0.05),而硝基苯组差异无统计学意义(P﹥0.05);硝基苯+苯胺组彗星拖尾率和彗星尾长均显著高于苯胺组。与硝基苯、苯胺组相比,硝基苯+苯胺组细胞内活性氧含量性显著增高,而谷胱甘肽含量显著降低(P﹤0.05)。[结论]硝基苯和苯胺及其混合物可显著影响SMMC-7721的细胞存活率,苯胺与硝基苯对SMMC-7721的细胞毒性作用可能存在着协同作用。硝基苯可能对肝癌细胞DNA断裂影响较小,苯胺和苯胺与硝基苯的混合物对细胞具有显著的DNA损伤作用,其毒性机制可能与细胞内活性氧增加、谷胱甘肽耗竭有关。其具体机制有待进一步研究。
[Objective] To study the combined toxic effects of aniline and nitrobenzene on SMMC-7721 hepatoma cells and provide a theoretical basis for better prevention of environmental compounds damage to human health. [Method] The established hepatocellular carcinoma cell line SMMC-7721 was treated with various concentrations of aniline, nitrobenzene and their mixtures (1: 1, c / c) for 24 hours. The cell viability, intracellular reactive oxygen species ) Content and glutathione (GSH) content, cell DNA strand breaks. [Result] After the cells were treated with aniline, nitrobenzene and their mixture for 24 h, the cell viability decreased with the increase of concentration, and the cell viability of the mixture group was significantly lower than that of the two substances treated alone. The aniline There may be a synergistic effect with nitrobenzene on the cytotoxicity of SMMC-7721. After treating with different concentrations of aniline, nitrobenzene and mixtures for 24 h, the comet cell ratio and comet tail length of aniline group, aniline + nitrobenzene group increased significantly compared with the negative control group (P <0.05) There was no significant difference in benzene group (P> 0.05). The tailing rate and comet tail length of comets in nitrobenzene + aniline group were significantly higher than those in aniline group. Compared with nitrobenzene and aniline group, nitrobenzene + aniline group significantly increased intracellular reactive oxygen species content, while glutathione content was significantly lower (P <0.05). [Conclusion] Nitrobenzene and aniline and their mixtures can significantly affect the cell viability of SMMC-7721. There may be synergistic effects of aniline and nitrobenzene on the cytotoxicity of SMMC-7721. Nitrobenzene may have little effect on the DNA fragmentation of hepatoma cells. The mixtures of aniline and aniline with nitrobenzene have a significant DNA damage effect on the cells. The toxicity mechanism may be related to the increase of intracellular reactive oxygen species and glutathione depletion. Its specific mechanism needs further study.