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目的:探讨褪黑素(MT)对噪声应激大鼠肝损伤的保护作用及可能机制.方法:将40只雄性SD大鼠随机分为5组:空白对照组、模型1组、模型2组、实验1组、实验2组,每组8只.实验组和模型组分别在应激前30min腹腔注射MT(15mg/kg)和等体积生理盐水.以56式冲锋枪射击时产生的120dB(A)的脉冲噪声,对实验组及模型组大鼠进行连续噪声刺激.噪声应激后,观察MT对各组大鼠血浆中皮质醇、超氧化物歧化酶(SOD),丙二醛(MDA)以及肝组织匀浆中SOD,MDA的影响.结果:肝脏病理检查显示模型组大鼠出现明显的病理性损伤,实验组大鼠损伤程度明显减轻;模型组大鼠血浆皮质醇、血浆及肝组织匀浆的MDA浓度较空白对照组显著升高,血浆及肝组织匀浆SOD活性显著降低(P<0.01),实验组大鼠血浆皮质醇的浓度、血浆及肝组织匀浆的MDA浓度较模型组显著降低,血浆及肝组织匀浆SOD活性显著升高(P<0.01).结论:MT能减轻噪声引起的大鼠肝损伤,其机制可能与抑制脂质过氧化反应有关.
Objective: To investigate the protective effect of melatonin (MT) on noise-induced liver injury in rats and its possible mechanism.Methods: Forty male SD rats were randomly divided into 5 groups: control group, model group 1, model group 2 , Experimental group 1 and experimental group 2, each group had 8. The experimental group and the model group were intraperitoneally injected with MT (15mg / kg) and normal saline 30min before the stress, respectively.The 120dB (A ), And the rats in the experimental group and the model group were stimulated by continuous noise.After noise stress, the effects of MT on plasma cortisol, superoxide dismutase (SOD), malondialdehyde (MDA) As well as the effects of SOD and MDA in liver tissue homogenate.Results: The liver pathological examination showed obvious pathological injury in the model group, and the injury degree in the experimental group was significantly reduced.The plasma cortisol, plasma and liver tissue in the model group The concentration of MDA in the homogenate was significantly higher than that in the blank control group, while the SOD activity in plasma and liver homogenate was significantly decreased (P <0.01). The concentration of plasma cortisol and the content of MDA in plasma and liver homogenate Group was significantly lower, SOD activity in plasma and liver homogenate was significantly increased (P <0.01) .Conclusion: MT Reduce noise-induced liver injury in rats, its mechanism may be related to the inhibition of lipid peroxidation.