姊妹染色单体交换(SCE)分析必须向细胞掺入Brdu,经特殊处理和染色而显示出 SCE 的变化。但Brdu 价格昂贵,且依赖进口。本文旨在探讨利用国产 Idu 取代进口 Brdu 进行 SCE 检测的可行性。材料与方法1.对象30名受试者全为健康献血员,男10名,女20名,平均年龄35岁。无吸烟史和1年内诱变剂和 x射线接触史。2.方法抽取外周血2ml,肝素抗凝,吸取0.3ml 全血接入5ml RPMI 1640培养基中,内含20%小牛血清和 Sister chromatid exchange (SCE) analysis must incorporate Brdu into the cells, with special treatment and staining to show changes in SCE. But Brdu is expensive and dependent on imports. This article aims to explore the feasibility of using domestic Idu to replace imported Brdu for SCE testing. Materials and Methods 1. Subjects 30 subjects were all healthy blood donors, 10 males and 20 females, mean age 35 years old. No smoking history and 1 year mutagen and x-ray exposure history. 2. Methods Peripheral blood 2ml, heparin anticoagulation, draw 0.3ml whole blood access 5ml RPMI 1640 medium, containing 20% ​​calf serum and