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探讨Ｐ１６基因突变在宫颈癌发生中的作用及基因突变的机制。利用点突变检测仪、水平和垂直板电泳对Ｐ１６基因的外显子１、外显子２的ＰＣＲ扩增产物作缺失和点突变分析。结果在２９例临床宫颈癌标本中有８例发生缺失突变，４例发生点突变，突变率约４１％。其中１例外显子１为不完全缺失突变即有低于３４３ｂｐ的扩增带。Ｐ１６基因的发生突变原因是因为其含有“ＣＧ”ＤＮＡ重复顺序，易发生ＤＮＡ重组及易位和重排。
To investigate the role of P16 gene mutation in cervical carcinogenesis and the mechanism of gene mutation. Point mutation detector, horizontal and vertical plate electrophoresis of P16 gene exon 1, exon 2 PCR amplification products for deletion and point mutation analysis. Results In 29 cases of cervical cancer specimens, 8 cases of deletion mutation occurred, 4 cases of point mutation, the mutation rate of about 41%. One case of exon 1 is incomplete deletion mutation that is less than 343bp amplification band. The reason for the mutation of P16 gene is that it contains “CG” DNA repetitive sequence, and DNA recombination and translocation and rearrangement are easy to occur.