目的通过离体与在体实验分别观察天麻素对长春新碱抑制乳腺癌细胞MCF-7生长和抗Walker-256乳腺癌荷瘤大鼠肿瘤生长作用的影响。方法体外培养乳腺癌细胞株MCF-7,采用MTT法检测不同剂量长春新碱对MCF-7细胞存活率的影响,计算长春新碱抑制乳腺癌细胞MCF-7生长的IC_(50),再测定单独给予不同浓度天麻素及其分别于IC_(50)长春新碱联合孵育24 h后MCF-7细胞生长的抑制率。通过SD大鼠在体皮下接种Walker-256乳腺癌细胞建立乳腺癌模型;实验分为A组:对照组;B组:乳腺癌肿瘤模型组;C组:乳腺癌肿瘤模型+长春新碱组;D、E组:乳腺癌肿瘤模型+长春新碱+60 mg/kg或120 mg/kg天麻素组。观察各组大鼠肿瘤生长情况,称瘤重计算抑瘤率,采用HE染色观察肿瘤切片组织形态学变化。结果长春新碱抑制乳腺癌细胞MCF-7生长的IC_(50)为28.34μg/mL;与空白对照组比较,不同浓度天麻素对MCF-7细胞的生存率无明显的改变(P>0.05);与单用长春新碱组比较,不同浓度天麻素与长春新碱联合给药对MCF-7细胞的生存率无明显的变化(P>0.05)。在在体实验中,与A组比较,B组大鼠肿瘤生长明显,瘤重达到32.76 7.65 g;与B组比较,C组肿瘤明显缩小,瘤重减轻到(19.30±4.24)g(P<0.05),抑瘤率41.09%;与C组比较,D、E组肿块进一步缩小,瘤重分别为(15.28±3.42)g、(10.99±2.87)g(P<0.05),抑瘤率分别达53.35%和66.44%。瘤体组织HE染色观察结果显示,B组肿瘤细胞核质比增高,核深染,可见病理性核分裂;与B组比较,C组核质比例减小,核呈圆形淡染,可见细胞坏死区域;与B组比较,D组中细胞坏死区域明显增大,可见部分肿瘤细胞核消失,E组细胞坏死进一步加重,可见片状凝固性坏死组织。结论天麻素对长春新碱抑制乳腺癌细胞MCF-7生长的作用无明显影响,但在体实验中天麻素能显著增强长春新碱对Walker-256荷瘤大鼠的抗肿瘤疗效,且呈剂量依赖性。 Objective To investigate the effects of gastrodin on the growth of MCF-7 breast cancer cells and on the growth of tumor-bearing rats with Walker-256 breast cancer by using vincristine in vitro and in vivo. Methods The breast cancer cell line MCF-7 was cultured in vitro. The effects of different doses of vincristine on the survival rate of MCF-7 cells were detected by MTT method. The IC50 of vincristine in inhibiting the growth of MCF-7 cells was calculated. The inhibitory rate of different concentrations of gastrodin and MCF-7 cell growth after 24 h incubation with IC50 (50) vincristine alone was given separately. Breast cancer model was established by subcutaneous inoculation of Walker-256 breast cancer cells in SD rats. The experiment was divided into A group: control group, B group: breast cancer model group, C group: breast cancer model + vincristine group, D, E group: breast cancer model + vincristine +60 mg / kg or 120 mg / kg gastrodin group. The growth of tumor in each group was observed, and the tumor inhibition rate was calculated by tumor weight. The histological changes of tumor tissue were observed by HE staining. Results Vincristine inhibited the growth of MCF-7 breast cancer cells with an IC 50 of 28.34μg / mL. Compared with the blank control group, the changes of survival rate of MCF-7 cells treated with different concentrations of gastrodin were not significantly different (P> 0.05) Compared with the single vincristine group, the combination of different concentrations of gastrodin and vincristine did not change the survival rate of MCF-7 cells (P> 0.05). Compared with group A, the tumor growth of group B was significantly higher than that of group A, and the tumor weight reached 32.76 7.65 g. Compared with group B, the tumor of group C was significantly reduced and the tumor weight was reduced to (19.30 ± 4.24) g (P < 0.05), and the tumor inhibition rate was 41.09%. Compared with group C, the tumors in groups D and E were further reduced and the tumor weights were (15.28 ± 3.42) g and (10.99 ± 2.87) g respectively (P <0.05) 53.35% and 66.44%. The results of HE staining showed that the ratio of nuclear to cytoplasm in group B was higher than that in group B, and stained nuclear stained. Compared with group B, Compared with group B, the area of ​​cell necrosis in group D increased obviously, some of the tumor nuclei disappeared, and the cell necrosis in group E was further aggravated. Conclusion Gastrodin has no effect on vincristine inhibiting the growth of MCF-7 breast cancer cells, but gastrodin can significantly enhance the anti-tumor effect of vincristine on Walker-256 tumor-bearing rats in vivo and in a dose-dependent manner Dependency.