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目的:分析SLE患者外周血中树突状细胞(DC)的表面标志,探讨DC和SLE发病之间的关系。方法:采用密度梯度离心法分离外周血单个核细胞(PBMC),在培养瓶中贴壁培养3h,吸去悬浮的细胞,联合应用GMCSF、IL4和TNFα刺激正常人及SLE患者外周血DC的增殖及分化成熟。用流式细胞仪分析DC的表面标志,用ELISA法检测培养9d的培养上清中IL12和IFNα的水平。结果:SLE患者DC表面CD1a、CD11c、CD40、CD83和CD123表达的阳性率,分别为:(58.88±7.64)%、(54.4±10.88)%、(37.29±8.08)%、(57.76±11.54)%和(13.14±4.44)%;健康志愿者(对照组)分别为:(47.71±4.01)%、(43.12±8.82)%、(28.59±7.07)%、(48.31±8.79)%和(9.85±3.97)%,两者相差明显(P<0.05)。SLE患者DC上CD80表达的阳性率为(55.16±10.12)%与对照组[(47.95±12.21)%]相差不明显(P>0.05)。培养上清中IL12的水平[(9.78±0.76)ng/L],较正常对照组[(7.49±0.74)ng/L]明显升高(P<0.05);SLE患者组IFNα的水平为[(2.95±0.61)ng/L]与对照组[(2.70±0.29)ng/L]相比升高不明显(P>0.05)。SLE患者非活动期与活动期培养上清中IL12和IFNα的水平无明显差异。结论:SLE患者的DC可能是通过其抗原递呈功能的增强及分泌IL12而参与本病的发病过程。
Objective: To analyze the surface markers of dendritic cells (DCs) in peripheral blood of patients with SLE and to explore the relationship between the pathogenesis of SLE and SLE. Methods: Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation and cultured in culture flasks for 3 hours. The suspended cells were aspirated and GMCSF, IL4 and TNFα were used in combination to stimulate the proliferation of peripheral blood mononuclear cells And mature differentiation. The surface markers of DCs were analyzed by flow cytometry and the levels of IL12 and IFNα in culture supernatants cultured for 9 days were detected by ELISA. RESULTS: The positive rates of CD1a, CD11c, CD40, CD83 and CD123 on the surface of SLE patients were (58.88 ± 7.64)%, (54.4 ± 10.88)%, (37.29 ± 8.08)% and (57.76 ± 11.54)%, respectively And (13.14 ± 4.44)%, respectively. The healthy volunteers (47.71 ± 4.01)%, (43.12 ± 8.82)%, (28.59 ± 7.07)%, (48.31 ± 8.79)% and )%, A significant difference between the two (P <0.05). The positive rate of CD80 expression in SLE patients was (55.16 ± 10.12)%, which was not significantly different from that in control group (47.95 ± 12.21%) (P> 0.05). Compared with normal control group [(7.49 ± 0.74) ng / L], the level of IL12 in culture supernatant was significantly higher than that in normal control group [(9.78 ± 0.76) ng / L] 2.95 ± 0.61 ng / L] was not significantly higher than that in the control group [(2.70 ± 0.29) ng / L] (P> 0.05). SLE patients inactive and active culture supernatant IL12 and IFNα levels no significant difference. CONCLUSIONS: DCs in SLE patients may participate in the pathogenesis of this disease through enhancement of their antigen presenting function and secretion of IL12.