论文部分内容阅读
为进一步揭示李属坏死环斑病毒(Prunus necrotic ringspot virus,PNRSV)新疆巴旦木分离物的分子特征、遗传变异及其与宿主之间的相互关系,采用RT-PCR方法扩增并克隆了4个PNRSV新疆巴旦木分离物运动蛋白(move protein,MP)基因片段,并进行了测序及序列同源性分析。结果表明,4个新疆巴旦木PNRSV分离物MP基因片段分别为259、258、254、260 bp;其核苷酸和氨基酸序列与已报道的PNRSV分离物的同源性分别为72.7%~91.7%和75.6%~92.9%,表现出明显差异,其中与美国分离物CH9同源性最高,分别达88.8%~91.7%和82.6%~92.9%;而与同属03亚组的苹果花叶病毒(Apple mosaic virus,Ap MV)同源性较低,仅为51.2%~58.1%和52.3%~61.9%;新疆PNRSV各分离株之间MP基因核苷酸序列同源性较高。系统发育树显示,4个新疆巴旦木PNRSV分离物与Ⅰ组代表毒株PV32的核苷酸序列同源性达88.4%~91.3%,并与Ⅰ组分离物聚集成簇,表明PNRSV新疆巴旦木分离物属于引起严重症状的Ⅰ组株系,且Ⅰ组中各分离物之间表现出一定的寄主相关性,而Ⅱ组和Ⅲ组中各分离物之间未表现出明显的寄主相关性。
To further reveal the molecular characteristics and genetic variation of Prunus necrotic ringspot virus (PNRSV) in Xinjiang and its relationship with hosts, the 4 A fragment of move protein (MP) gene of Xinjiang Pasteurella multocida from PNRSV was sequenced and its sequence homology was analyzed. The results showed that the MP gene fragments of four Xinjiang PNRSV isolates were 259, 258, 254 and 260 bp, respectively. The nucleotide and amino acid sequences of the two strains shared 72.7% -91.7 % And 75.6% -92.9%, respectively. The results showed that CH9 shared the highest homology with American isolates, which ranged from 88.8% to 91.7% and 82.6% to 92.9% Apple mosaic virus (Ap MV)) was only 51.2% -58.1% and 52.3% -61.9%, respectively. The homology of the nucleotide sequence of MP gene among the isolates of Xinjiang PNRSV was high. Phylogenetic tree showed that the nucleotide sequence homology of four Xinjiang strains isolated from PNRSV with that of group Ⅰ was 88.4% -91.3%, which was clustered with the group Ⅰ isolates, indicating that PNRSV Xinjiang bar Dan-wood isolates belonged to group Ⅰ which caused serious symptoms, and some isolates in group Ⅰ showed some host-relatedness, but no significant host-related changes were found among the isolates in groups Ⅱ and Ⅲ Sex.