论文部分内容阅读
本文用图像分析的方法对正常胃粘膜、轻、中、重度异型增生、管状腺癌及低分化腺癌共30例石蜡切片标本的细胞核仁组成区嗜银蛋白(AgNOR)和细胞核DNA含量进行了定量检测,并进行了两者的相关性研究。结果显示AgNOR颗粒数量及DNA含量均随病变程度加重而增加,AgNOR数量与DNA含量、超二倍体及超四倍体细胞百分数、DNA直方图倍体类型之间有明显的相关性(r=0.8811,0.7288,0.8606,0.8793);提示AgNOR数量与DNA含量的变化对胃癌前病变的判断与研究具有相近的意义。与细胞核DNA含量检测相比,AgNOR计数分析具有染色简便,易于推广的优点,是胃癌及癌前病变研究中一项新的、实用的参考指标。
In this paper, AgNOR and nuclear DNA content of nucleolar constituents in 30 cases of normal gastric mucosa, mild, moderate, severe dysplasia, tubular adenocarcinoma, and poorly differentiated adenocarcinoma were studied by image analysis. Quantitative testing, and conducted a correlation study between the two. The results showed that the number of AgNOR particles and DNA content increased with the severity of the lesion, and there was a significant correlation between AgNOR counts and DNA content, the percentage of hyperdiploid and hypertetraploid cells, and the histogram of DNA histograms (r= 0.8811,0.7288,0.8606,0.8793); suggest that changes in the number of AgNOR and DNA content have similar significance for the judgment and study of precancerous lesions. Compared with the detection of nuclear DNA content, AgNOR count analysis has advantages of simple staining and easy popularization. It is a new and practical reference index for the study of gastric cancer and precancerous lesions.