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目的 建立与人类原发性肝癌相似的大鼠肝癌模型 ,探讨其薄层螺旋CT动态表现。方法 实验组大鼠自由饮用含DE NA水 1 0 0d。通过剖腹探查、病理切片证实诱发肝癌成功。通过血清学ALT、AST、γ -GT、ALP、BIL、ALB、A/G和PT检查 ,证实实验大鼠的肝功受损情况及肝硬化背景。通过薄层螺旋CT动态增强扫描 ,了解诱发性肝癌的血供特征。结果 大鼠饮用DENA水后 ,肝癌发生率 1 0 0 %。血清ALT、AST、γ -GT、ALP、BIL升高 ,与临床肝硬化病人的血清学变化十分近似。薄层螺旋CT扫描诱发性肝癌动脉期早期 (0～ 1 0s)明显强化 ,并迅速衰减 ,呈“快进快出”表现 ,与人类肝癌表现十分相似。于 0～ 1 0s之间 ,肝癌与肝脏之间形成了最大的密度差 ,CT扫描最有价值。结论 DENA诱发性肝癌模型 ,成功率高 ,与人类原发性肝癌具有十分相似的肝硬化背景及血供特点 ,能为肝癌血管介入及新生血管抑制剂研究提供良好的动物模型
Objective To establish a rat model of hepatocellular carcinoma (HCC) resembling human primary hepatocellular carcinoma (HCC) and investigate its dynamic manifestation. Methods Rats in experimental group were allowed to drink DEDA water for 10 days. Through laparotomy, pathology confirmed the successful induction of liver cancer. Liver damage and liver cirrhosis were confirmed by serological ALT, AST, γ-GT, ALP, BIL, ALB, A / G and PT. By thin-slice spiral CT dynamic enhanced scan to understand the characteristics of blood supply induced liver cancer. Results After drinking DENA water in rats, the incidence of liver cancer was 100%. Serum ALT, AST, γ-GT, ALP, BIL increased, and clinical cirrhosis patients serological changes are very similar. The early stage of arterial phase (0 ~ 10s) in thin-slice spiral CT-scan-induced hepatocellular carcinoma significantly enhanced and rapidly decayed, showing “fast-forward-fast-forward” performance, which is very similar to that of human hepatocellular carcinoma. Between 0 ~ 10s, the largest density difference between liver cancer and liver was formed, and CT scan was the most valuable. Conclusion DENA-induced hepatocellular carcinoma model has a high success rate and has very similar liver cirrhosis background and blood supply characteristics to human primary hepatocellular carcinoma. It can provide a good animal model for the study of vascular intervention and neovascularization inhibitors