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植物名称:甜樱桃(Prunus avium)。材料类别:纳翁(Napo leon)、大紫(BlackTartarin)、保加利亚品种两个(、xespyc)、红灯等品种的茎尖及侧芽。培养条件:基本培养基为MS。①芽分化培养基每升附加BA0.5—1.0毫克;吲哚乙酸0.2毫克;蔗糖30克。②生根培养基为1/2 MS(大量元素),其它成分同MS培养基,每升附加吲哚乙酸1—3毫克;蔗糖 20克;琼脂 5克。③培养条件:25±2℃,光照强度1500lx;24小时光照。生长与分化情况:接种10天后即可见到茎尖明
Plant Name: Sweet Cherry (Prunus avium). Material categories: Napoleon, Black Tartarin, Bulgarian two (xespyc), red light and other varieties of shoot tips and lateral buds. Culture conditions: The basic medium for MS. ① bud differentiation medium added BA0.5-1.0 mg per liter; indole acetic acid 0.2 mg; sucrose 30 grams. ② rooting medium for 1/2 MS (a large number of elements), the other ingredients with MS medium, add 1-3 mg of indole acetic acid per liter; 20 grams of sucrose; agar 5 grams. ③ culture conditions: 25 ± 2 ℃, light intensity 1500lx; 24 hours light. Growth and differentiation: 10 days after inoculation to see the tip sharp