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目的探讨白细胞介素６（ＩＬ－６）在老年女性原发性骨质疏松症（ＯＰ）发病机理中的作用。方法采用ＩＬ－６依赖性细胞株Ｂ９．９增殖反应四甲基偶氮唑蓝（ＭＴＴ）比色法检测９７例不同年龄组健康女性血清ＩＬ－６活性以及骨密度的水平。结果随年龄的增长，女性的ＩＬ－６活性逐渐升高（老年组、老年前期组分别为７．８±３．２及３．６±２．６Ｕ／ｍｌ，青中年组未达到２Ｕ／ｍｌ），骨密度值逐渐下降（３组分别为０．７３±０．１１、０．８９±０．１５及０．９９±０．１６ｇ／ｃｍ２），两者呈明显负相关（ｒ＝－０．７２４，Ｐ＜０．０５）；绝经后女性的ＩＬ－６活性较绝经前女性明显升高（分别为８．４±３．６及２．８±１．２Ｕ／ｍｌ，Ｐ＜０．０５），而骨密度值却明显降低（分别为０．６９±０．１８及１．０２±０．１６ｇ／ｃｍ２，Ｐ＜０．０５）。结论ＩＬ－６活性的升高与绝经后女性的骨丢失有关，ＩＬ－６可能参与了ＯＰ的发病机理。
Objective To investigate the role of interleukin-6 (IL-6) in the pathogenesis of elderly primary osteoporosis (OP). Methods Serum levels of IL-6 and bone mineral density (BMD) were measured in 96 healthy women with different age groups by MTT assay using B9.9 proliferation assay. Results With age, the activity of IL-6 in women gradually increased (7.8 ± 3.2 and 3.6 ± 2.6U / ml in the elderly group and the elderly group, and 2U / ml), the bone mineral density decreased gradually (0.73 ± 0.11,0.89 ± 0.15 and 0.99 ± 0.16g / cm2 respectively in the three groups), and there was a significant negative correlation between them (r = - 0.724, P <0.05). IL-6 activity in postmenopausal women was significantly higher than that in premenopausal women (8.4 ± 3.6 vs 2.8 ± 1.2 U / ml, P <0. .05), while the BMD was significantly lower (0.69 ± 0.18 and 1.02 ± 0.16g / cm2, respectively, P <0.05). Conclusions Elevated IL-6 activity is associated with bone loss in postmenopausal women. IL-6 may be involved in the pathogenesis of OP.