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目的研究芪参益气滴丸(QS)对肾性高血压大鼠左室肥厚及磷酸化细胞外信号调节激酶1/2(p-ERK1/2)表达的影响。方法选择50只体质量200 g左右的雄性Wistar大鼠,随机分为5组(n=10),建立两肾一夹肾血管性高血压大鼠左室肥厚模型,包括:假手术组(Sham)、手术组(2K1C)、QS小剂量干预组、QS大剂量干预组、缬沙坦干预组。术后4周末开始灌胃,QS大、小剂量干预组分别给予QS 250、125 mg/(kg.d),缬沙坦干预组给予缬沙坦30 mg/(kg.d),持续8周;假手术组和手术组给予相同剂量生理盐水灌胃。治疗8周后终止实验,测量大鼠尾动脉收缩压(SBP)、ELISA法检测抗氧化酶(SOD和GSH-PX)和活性氧自由基(ROS)以及用免疫组化法和Western印迹方法检测大鼠左室心肌p-ERK1/2蛋白的表达。结果术后12周,手术组和QS大、小剂量组收缩压(SBP)、左心室质量指数(LVMI)与假手术组相比,均显著升高(P<0.01),而与手术组相比,QS大、小剂量组LVMI显著降低,SOD和GSH-PX的活性升高,ROS以及p-ERK1/2蛋白的表达降低,但对大鼠收缩压无显著影响。结论 QS对肾性高血压大鼠左室肥厚有抑制作用,其机制可能与其具有抗氧化活性以及下调心肌p-ERK1/2表达相关。
Objective To investigate the effects of Qishen Yiqi Dripping Pill (QS) on left ventricular hypertrophy and the expression of phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1 / 2) in renal hypertensive rats. Methods Fifty male Wistar rats with body weight of 200 g were randomly divided into five groups (n = 10) to establish a model of left ventricular hypertrophy in two-kidney-one-folder renovascular hypertensive rats, including sham operation group ), Operation group (2K1C), QS low-dose intervention group, QS high-dose intervention group, valsartan intervention group. At the end of the fourth week, the rats were started gavage. QS 250,125 mg / (kg · d) were administered to QS large and small dose intervention groups. Valsartan 30 mg / (kg.d) was given to valsartan intervention group for 8 weeks ; Sham-operation group and operation group were given the same dose of normal saline. After 8 weeks of treatment, the experiment was terminated, the tail artery systolic pressure (SBP), antioxidant enzymes (SOD and GSH-PX) and reactive oxygen species (ROS) were detected by ELISA and detected by immunohistochemistry and Western blotting Expression of p-ERK1 / 2 protein in left ventricular myocardium of rats. Results Compared with the sham-operated group, the systolic blood pressure (SBP) and left ventricular mass index (LVMI) of the operation and QS groups were significantly increased at 12 weeks after operation (P <0.01) Compared with the QS group, the LVMI was significantly decreased and the activities of SOD and GSH-PX were increased, the expressions of ROS and p-ERK1 / 2 were decreased, but the systolic pressure was not significantly affected. Conclusion QS can inhibit left ventricular hypertrophy in renovascular hypertensive rats. The mechanism may be related to its anti-oxidative activity and down-regulation of p-ERK1 / 2 expression.