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为探讨类风湿关节炎(rheumatoid arthritis,RA)患者PBMC中miR-146a、miR-155、Ets-1和IRAK1的表达水平及与病情活动度的相关性,收集RA高活动期患者23例、低活动期患者21例和健康志愿者22例,采用qRT-PCR检测PBMC中miR-146a、miR-155、Ets-1、IRAK1的相对表达水平,同时分析其与临床及实验室指标的相关性。RA患者PBMC的miR-146a、miR-155相对表达量显著高于健康对照组(P<0.05),高活动组高于低活动组(P<0.05)、低活动组与健康对照组差异无统计学意义(P>0.05);RA患者PBMC的Ets-1表达水平与健康对照组差异无统计学意义,高活动组显著低于低活动组和健康对照组(P<0.05)、低活动组与健康对照组差异无统计学意义(P>0.05);RA患者PBMC的IRAK1表达水平显著低于健康对照组(P<0.05),高活动组显著低于健康对照组(P<0.01)、低活动组显著低于健康对照组(P<0.05),而高活动组与低活动组间无统计学差异(P>0.05)。在RA患者中miR-155表达水平与血沉正相关(r=0.319,P=0.042)而与血红蛋白负相关(r=-0.386,P=0.017);Ets-1与CRP负相关(r=-0.408,P=0.007);IRAK1与RF、DAS 28、总补体负相关(r=-0.513,P=0.001;r=-0.332,P=0.029;r=-0.49,P=0.015)。研究结果显示,RA高活动组患者PBMC中miR-146a、miR-155表达升高,Ets-1、IRAK1表达降低,可能参与了RA发病过程的调控;但本研究样本量有限,miR-146a、miR-155和Ets-1、IRAK1参与RA发病的关系有待后续大样本研究证实,且相关调控机制仍需进一步深入研究。
To investigate the expression of miR-146a, miR-155, Ets-1 and IRAK1 in PBMC of patients with rheumatoid arthritis (RA) and its correlation with disease activity, 23 patients with high RA activity were collected, The activity of miR-146a, miR-155, Ets-1 and IRAK1 were detected by qRT-PCR in 21 active patients and 22 healthy volunteers. The correlation between them and clinical and laboratory parameters was also analyzed. The relative expression levels of miR-146a and miR-155 in PBMCs of RA patients were significantly higher than those in healthy controls (P <0.05), those in high activity group were significantly higher than those in low activity group (P <0.05), while there was no significant difference between low activity group and healthy control group (P> 0.05). The expression of Ets-1 in PBMC of RA patients was not significantly different from that in healthy controls (P <0.05), and the activity of Ets-1 in RA patients was significantly lower than that in low activity group and healthy control group The expression of IRAK1 in PBMC of RA patients was significantly lower than that in healthy controls (P <0.05), while the activity of IRAK1 in high activity group was significantly lower than that in healthy control group (P <0.01) Group was significantly lower than the healthy control group (P <0.05), while there was no significant difference between high activity group and low activity group (P> 0.05). The level of miR-155 in RA patients was positively correlated with ESR (r = 0.319, P = 0.042) and negatively correlated with hemoglobin (r = -0.386, P = 0.017) , P = 0.007). IRAK1 was negatively correlated with RF and DAS28 total complement (r = -0.513, P = 0.001; r = -0.332, P = 0.029; r = -0.49, P = 0.015). The results showed that the expression of miR-146a and miR-155, and the expression of Ets-1 and IRAK1 in PBMC of patients with high RA activity were decreased, which may be involved in the regulation of RA pathogenesis. However, The relationship between miR-155, Ets-1 and IRAK1 in the pathogenesis of RA needs to be confirmed in the follow-up large sample studies, and the relevant regulatory mechanisms still need further study.