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目的:研究咪喹莫特抑制兔耳瘢痕增生的作用及可能机制。方法:选取10只新西兰大耳白兔,雌雄不限,根据本实验室的改良方法建立兔耳瘢痕模型,每只兔耳腹侧做六个直径为1cm的圆形创面,每个相距1.5cm,双侧对称,左耳为实验组涂抹5%咪喹莫特软,右耳为对照组涂抹等量凡士林软膏,待术后14天上皮化均完全后开始涂抹,一日一次。分别于术后14天、21天、28天、35天及42天同一时间点空气栓塞随机处死2只兔子,后沿每个瘢痕边缘外周的0.5cm处环形切下,经瘢痕最凸点直径一切为二,一半固定后行苏木精-伊红(HE)染色及Masson三色法染色,观察形态学差异,测量并计算瘢痕增生指数(Scar elevation index,SEI);另一半用于提取RNA,反转录后行Real-time PCR检测细胞外基质Ⅰ型胶原(Collagen-Ⅰ,Col-Ⅰ),细胞因子IFN-γ及IL-4的表达。结果:HE及Masson染色可见实验组胶原沉积较对照组明显减少,SEI显示空白对照组于术后第21天增生程度达到高峰,其增生程度明显高于实验组;Real-time PCR结果可见实验组Col-Ⅰ及IL-4的表达在各时间点明显降低,且IFN-γ的表达较对照组增高。结论:咪喹莫特可能通过调节IFN-γ及IL-4的表达来发挥抑制瘢痕增生的作用。
Objective: To study the effect of imiquimod on the inhibition of rabbit ear scar proliferation and its possible mechanism. Methods: Ten New Zealand white rabbits were selected and were male or female. Rabbit ear scar models were established according to the improved method in our laboratory. Six rabbit wounds with a diameter of 1 cm , Bilateral symmetry, the left ear for the experimental group smear 5% imiquimod soft, the right ear as the control group smear the same amount of Vaseline ointment until 14 days after the epithelialization were completely smear, once a day. Two rabbits were randomly sacrificed at 14, 21, 28, 35 and 42 days after air embolization respectively. The rabbits were excised circularly along the periphery of each scar at 0.5cm. The maximum diameter of the scar All of them were fixed in two and one half, hematoxylin and eosin (HE) staining and Masson’s trichrome staining were used to observe the morphological differences, and scar elevation index (SEI) was measured and calculated. The other half was used to extract RNA After transfection, Real-time PCR was used to detect the expression of collagen Ⅰ, collagen Ⅰ and cytokines IFN-γ and IL-4. Results: HE and Masson staining showed that collagen deposition in the experimental group was significantly reduced compared with the control group, SEI showed that the proliferation of the control group reached a peak at 21 days after operation, and the degree of hyperplasia was significantly higher than that of the experimental group. Real-time PCR results showed that the experimental group The expression of Col-I and IL-4 were significantly decreased at each time point, and the expression of IFN-γ was higher than that of the control group. Conclusion: Imiquimod may inhibit the proliferation of scar by regulating the expression of IFN-γ and IL-4.