土壤中的反硝化作用由于直接影响到肥料氮的利用率和环境问题，仍然是氮素研究领域的热点和难点之一，而反硝化作用研究的进展在很大程度上依赖与土壤反硝化的田间测定方法的建立。文章就目前反硝化研究领域常用的１５Ｎ平衡差值法、１５Ｎ示踪气体通量法、乙炔抑制气室法、乙炔抑制土柱法的原理、气体样的采集、测定和计算作了综述，以期为土壤反硝化的研究提供依据。 The denitrification in soil is still one of the hot and difficult issues in the field of nitrogen research due to the direct impact on fertilizer nitrogen utilization and environmental issues. The progress of denitrification research is largely dependent on the relationship between soil denitrification Establishment of field measurement methods. In this paper, the current 15N equilibrium difference method, 15N tracer gas flux method, acetylene gas chamber inhibition method, acetylene inhibition soil column method, gas sampling, determination and calculation are reviewed in this paper. Provide the basis for the research of soil denitrification.