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目的： 大量获得纯化的日本血吸虫２２６ ｋ Ｄａ 重组抗原。方法： 用 Ｐ Ｃ Ｒ 方法将其编码基因序列经改造后亚克隆入载体质粒ｐ Ｇ Ｅ Ｘ１λ Ｔ 进行表达。结果： 得到了融合表达的蛋白抗原。此融合蛋白表达量大， 用凝血酶酶切后易大量制备纯化的重组２２６ ｋ Ｄａ 抗原。结论： 以此融合蛋白免疫的小鼠抗血清进行免疫印迹试验， 表明 Ｓｊ２２６／ Ｓｊ２６ Ｇ Ｓ Ｔ 融合重组蛋白具有与 Ｓｊ２２６ 蛋白一样的免疫学活性。
Objective: To obtain a large amount of purified 22.6 kDa recombinant antigen of Schistosoma japonicum. Methods: The coding sequence of the gene was subcloned into vector pG E X1λ T by PCR. Results: The expressed fusion protein was obtained. The fusion protein expressed large amount, easy to use thrombin easily prepared recombinant recombinant 22 6 k Da antigen. CONCLUSION: The results of Western blotting showed that the Sj22.6 / Sj26G S T fusion recombinant protein has the same immunological activity as Sj22.6 protein.