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[目的]研究苹果多酚对糖尿病小鼠血脂、血糖水平和肝、肾、脂肪组织PPARγmRNA表达的影响。[方法]雄性小鼠分为对照组(n=12)、模型组(n=12)和苹果多酚干预组(n=36)。通过给予高脂高糖饲料喂养,腹腔注射链脲佐菌素,建立糖尿病小鼠模型。应用苹果多酚对糖尿病小鼠干预治疗60 d。在试验结束时,集中处死鼠,搜集血标本和肝、肾、脂肪组织标本。检测各组大鼠的血MDA、FBG、FINS、TG、TC等浓度。采取RT-PCR方法,检测各组小鼠肝、肾、脂肪组织PPARγmRNA的表达。[结果]与Con组相比,模型组小鼠血清MDA、血脂、血糖、胰岛素水平显著升高(P<0.01),与模型组相比,苹果多酚能显著降低小鼠的血清MDA、血脂、血糖、胰岛素水平(P<0.05)。与Con组相比,模型组小鼠肝、肾、脂肪组织PPARγmRNA表达水平显著下降,苹果多酚组小鼠肝、肾、脂肪组织PPARγmRNA的表达水平较模型组均有明显增加(P<0.05)。[结论]苹果多酚能够降低糖尿病小鼠血清MDA、血脂、血糖水平,减轻胰岛素抵抗,提高PPARγmRNA的表达。苹果多酚可能发挥着类似PPARγ激动剂的效用,通过提高PPARγmRNA的表达来调节糖尿病小鼠的血糖、血脂代谢。
[Objective] The research aimed to study the effects of apple polyphenols on blood lipid, blood glucose level and PPARγ mRNA expression in liver, kidney and adipose tissue of diabetic mice. [Methods] Male mice were divided into control group (n = 12), model group (n = 12) and apple polyphenols intervention group (n = 36). By feeding high-fat and high-sugar diet, intraperitoneal injection of streptozotocin, diabetic mouse model was established. Application of apple polyphenols on diabetic mice for 60 days. At the end of the experiment, mice were sacrificed and blood samples and liver, kidney and adipose tissue samples were collected. The concentrations of MDA, FBG, FINS, TG and TC in the blood of each group were detected. The expression of PPARγmRNA in liver, kidney and adipose tissue of each group was detected by RT-PCR. [Results] Compared with the Con group, the levels of serum MDA, blood lipids, blood glucose and insulin in the model group were significantly increased (P <0.01). Compared with the model group, apple polyphenols could significantly reduce the serum MDA, , Blood glucose, insulin (P <0.05). Compared with the Con group, the expression of PPARγmRNA in the liver, kidney and adipose tissue of the model group decreased significantly. Compared with the model group, the PPARγ mRNA expression in the liver, kidney and adipose tissue of the apple polyphenols group increased significantly (P <0.05) . [Conclusion] Apple polyphenols can reduce serum MDA, blood lipid, blood glucose level, reduce insulin resistance and increase the expression of PPARγmRNA in diabetic mice. Apple polyphenols may play a similar PPARγ agonist effect, by increasing the expression of PPARγ mRNA to regulate blood glucose and lipid metabolism in diabetic mice.