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将腹腔注射地西洋（7．5mg／kg）而镇静的SD大鼠，置于体积描记器，测定其自主呼吸变化。吸入氯化乙酰甲胆碱（MCh）和氯化乙酰胆碱（ACh）气雾对呼吸幅度无明显影响，可浓度依赖性地增加呼吸频率，且两药的作用强度相似，但MCh作用维持11min，ACh仅维持3min。乌拉坦麻醉可抑制呼吸和对MCh的反应；硫酸阿托品、硫酸沙丁腹醇和氨茶碱抑制MCh引起的频率增快：吸入抗原气雾后6h能增强致敏大鼠对MCh的敏感性。实验表明，按本方法用地西泮镇静和吸入MCh是测定大鼠气道反应性的理想条件，呼吸频率增快可作为气道反应性的指标。
Sprague - Dawley (7.5 mg / kg) sedation SD rats were placed on a plethysmograph and their spontaneous breathing changes were measured. Inhalation of methacholine chloride (MCh) and acetylcholine chloride (ACh) had no significant effect on the respiratory rate, but increased the respiratory rate in a concentration-dependent manner. Only maintain 3min. The urethane anesthesia can inhibit the respiration and the response to MCh; Atropine sulfate, Sulfobarbital and aminophylline inhibition of MCh caused by increased frequency: 6h inhalation of antigen aerosols can sensitize rats sensitized to MCh. Experiments show that, according to this method with diazepam and inhaled MCh is an ideal condition for the determination of rat airway responsiveness, respiratory rate can be used as an index of airway responsiveness.