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目的探讨受体结合肿瘤抗原(RCAS1)、血管内皮生长因子(VEGF)和基质金属蛋白酶-9(MMP-9)在早期宫颈浸润癌组织中的表达及其与宫颈癌组织侵袭、转移的关系。方法采用免疫组织化学 S-P 法,分别检测 RCAS1、VEGF 及 MMP-9蛋白在95例早期宫颈浸润癌(ICC),82例宫颈上皮内瘤样病变(CIN)和24例正常宫颈上皮(NCE)中的表达,并分析其与宫颈癌临床病理学参数间的关系及3哲间的相关性。结果免疫组织化学方法显示,从 NCE 到 CIN 再到 ICC,RCAS1蛋白阳性表达率分别为0、39.0%和72.6%;VEGF 蛋白阳性表达率分别为16.7%、41.5%和67.4%;MMP-9蛋白阳性表达率分别为18.2%、53.7%和78.9%,差异有统计学意义(P<0.01)。RCAS1在 ICC 中的表达与盆腔淋巴结转移、脉管浸润、间质浸润和组织学分级有关(P<0.01);而与年龄和组织学类型、临床分期无关(P>0.05);MMP-9和 VEGF 在 ICC 中的阳性表达与盆腔淋巴结转移、脉管浸润、间质浸润和临床分期有关(P<0.05),与年龄、绀织学分级和组织学类型无关(均 P>0.05)。在宫颈癌组织中,RCAS1、VEGF 和 MMP-9蛋白表达均呈显著正相关(均 P<0.01)。结论 RCAS1、VEGF 和MMP-9蛋白异常表达在宫颈癌的恶化、侵袭、转移中起重要作用,联合检测这些指标并分析其表达的相互关系有助于判断宫颈癌的转移潜能及指导术后,干预治疗。
Objective To investigate the expression of receptor-binding tumor antigen (RCAS1), vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) in early invasive cervical carcinoma and their relationship with invasion and metastasis of cervical cancer. Methods Immunohistochemical SP method was used to detect the expression of RCAS1, VEGF and MMP-9 in 95 cases of early invasive cervical cancer (ICC), 82 cases of cervical intraepithelial neoplasia (CIN) and 24 cases of normal cervical epithelium (NCE) The relationship between clinicopathological parameters and clinicopathological parameters was analyzed. Results Immunohistochemistry showed that the positive expression rates of RCAS1 protein from NCE to CIN to ICC were 0, 39.0% and 72.6%, respectively. The positive rates of VEGF protein were 16.7%, 41.5% and 67.4%, respectively. The expressions of MMP-9 protein The positive rates were 18.2%, 53.7% and 78.9%, respectively, with significant difference (P <0.01). The expression of RCAS1 in ICC was correlated with pelvic lymph node metastasis, vascular invasion, interstitial infiltration and histological grade (P <0.01), but not with age, histological type and clinical stage (P> 0.05) The positive expression of VEGF in ICC was correlated with pelvic lymph node metastasis, vascular invasion, interstitial infiltration and clinical stage (P <0.05), but not with age, histological grade and histological type (all P> 0.05). In cervical cancer, RCAS1, VEGF and MMP-9 protein expression showed a significant positive correlation (all P <0.01). Conclusion The abnormal expression of RCAS1, VEGF and MMP-9 may play an important role in the progression, progression and metastasis of cervical cancer. The combined detection of these indicators and the analysis of the correlation between them may be helpful in judging the metastatic potential of cervical cancer and guiding the postoperative, Intervention treatment.