论文部分内容阅读
目的探讨运用16S rRNA基因检测技术在快速诊断自发性腹膜炎的临床价值。方法针对16S rRNA区域的恒定区,设计一条通用引物,对已知病原菌、人类基因组DNA、巨细胞病毒和白色假丝酵母菌进行PCR扩增,检验方法的特异性,用倍比稀释法检测方法的灵敏性。然后用此方法检测自发性腹膜炎患者的腹水样本,并与培养法进行比较分析。结果已知病原菌均扩增出了特异产物,人类基因组DNA、巨细胞病毒和白色假丝酵母菌无相对应产物,PCR敏感性可达1 pg大肠杆菌DNA。69例腹水样本PCR方法检测阳性率为33.3%,培养法阳性率为14.5%,PCR法阳性率显著高于培养法(P<0.05)。结论 16S rRNA基因检测技术具有检测时间短、敏感性高、结果准确可靠等优点,具有重要的临床应用价值。
Objective To explore the clinical value of 16S rRNA gene detection in the rapid diagnosis of spontaneous peritonitis. Methods Aiming at the constant region of 16S rRNA region, a universal primer was designed to amplify the known pathogens, human genomic DNA, cytomegalovirus and Candida albicans. The specificity of the method was tested by the method of multiplier dilution Sensitivity. Then this method was used to detect ascites in patients with spontaneous peritonitis and compared with the culture method. Results It was known that specific products were amplified from pathogenic bacteria. Human genomic DNA, cytomegalovirus and Candida albicans had no corresponding products, and the sensitivity of PCR was up to 1 pg of E. coli DNA. The positive rate of PCR was 33.3% in 69 ascites samples, 14.5% in culture method, and the positive rate of PCR method was significantly higher than that of culture method (P <0.05). Conclusion 16S rRNA gene detection technology has the advantages of short detection time, high sensitivity, accurate and reliable results, and has important clinical value.