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目的 探讨各种细胞因子对Ｔ细胞生长激素（ＧＨ）基因表达的影响。方法 构建含人ＧＨ调控序列的荧光素酶报告基因质粒 ＰＧＬ２－ＧＨ－ｌｕｃ，然后转染入Ｔ淋巴细胞系 Ｊｕｒｋａｔ Ｅ６－１细胞中，在培养液中分别加入各种细胞因子。结果 生理浓度的ＩＬ－１β、ＴＮＦ－β和 ＩＦＮ－γ，对 Ｊｕｒｋａｔ细胞中荧光素酶的表达具有抑制作用（Ｐ＜０.０５）。结论 细胞因子参与了调节淋巴细胞 ＧＨ基因的表达。
Objective To investigate the effect of various cytokines on the expression of growth hormone (GH) gene in T cells. Methods The luciferase reporter gene plasmid PGL2-GH-luc containing human GH regulatory sequence was constructed and transfected into T lymphocyte cell line Jurkat E6-1. Various cytokines were added into the culture medium. Results The physiological concentrations of IL-1β, TNF-β and IFN-γ inhibited the expression of luciferase in Jurkat cells (P <0.05). Conclusion Cytokines are involved in the regulation of lymphocyte GH gene expression.