论文部分内容阅读
目的研究神经突触前膜胞内蛋白(Munc18)抗体致大鼠的机制。方法60只SD大鼠分为4组,在实验组大鼠的海马CA1区间断注射Munc18抗体,共9次。监测大鼠的脑电图和样行为。10周后全部大鼠处死取脑,切片做HE、尼氏和TUNEL染色后观察。结果实验组12只大鼠中,10只出现异常脑电,5只在6周之后仍然存在;9只出现1~4级不等的样行为,6周后仍有4只存在样行为;大鼠脑片显示海马区神经细胞数量明显减少(155±20,P<0·01),而凋亡细胞数量明显增多(16·80±3·32,P<0·01),伴有胶质细胞增生及细胞形态异常。结论Munc18抗体能慢性点燃致大鼠,其机制可能与其诱导整个海马区神经细胞的凋亡和丢失有关。
Objective To investigate the mechanism of pre-synaptic membrane protein (Munc18) antibody in rats. Methods Sixty SD rats were divided into four groups. The Munc18 antibody was injected into the hippocampal CA1 region of rats in the experimental group for 9 times. EEG and -like behaviors were monitored in rats. After 10 weeks, all rats were sacrificed and brain sections were taken for HE, Nissl and TUNEL staining. Results Of the 12 rats in the experimental group, 10 had abnormal EEGs, and 5 remained after 6 weeks. Nine animals showed unequal 1-like behaviors from 1 to 4, and 4 animals remained 6-like after 6 weeks The number of apoptotic cells in hippocampus of rats was significantly decreased (155 ± 20, P <0.01) and the number of apoptotic cells was significantly increased (16 · 80 ± 3.32, P <0.01) Glial cell proliferation and abnormal cell morphology. Conclusion The Munc18 antibody can cause chronic ignition of rats and its mechanism may be related to its induction of apoptosis and loss of nerve cells in the whole hippocampus.