论文部分内容阅读
目的 探讨温热 ＣＤＤＰ对 Ｈｃａ－Ｆ细胞的生长抑制、温度与药效的关系以及诱导凋亡的作用。方法 采用ＭＴＴ法，透射电镜，ＤＮＡ电泳以及流式细胞术分析法。结果ＣＤＤＰ对Ｈｃａ－Ｆ细胞有明显的细胞毒性，其ＩＣ５０值为０．５７μｇ／ｍｌ。ＣＤＤＰ合并高温（＞４１℃）加热明显提高了ＣＤＤＰ的杀伤力，二者之间存在着协同增敏作用。其作用强度呈现对药物浓度、作用时间与加热温度的正相关性，且在一定浓度范围内以谤导肿瘤细胞凋亡的发生为主。结论 加热（＞４１℃）顺铂提高了顺铂的细胞毒性。诱导细胞凋亡的发生是其抗肿瘤作用的主要机制之一。
Objective To investigate the effects of warm CDDP on the growth of Hca-F cells, the relationship between temperature and pharmacodynamics and the induction of apoptosis. Methods MTT method, transmission electron microscopy, DNA electrophoresis and flow cytometry analysis. Results CDDP had obvious cytotoxicity on Hca-F cells with IC50 of 0.57μg / ml. CDDP combined with high temperature (> 41 ℃) heating significantly increased the lethality of CDDP, there is synergistic sensitization between the two. The intensity of action showed a positive correlation between the drug concentration, the action time and the heating temperature, and in a certain concentration range, the occurrence of tumor cell apoptosis was the mainstay. Conclusion Heating (> 41 ° C) cisplatin increases cisplatin cytotoxicity. Induction of apoptosis is one of the main mechanisms of its anti-tumor effect.