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目的测定糖尿病大鼠骨折后骨痂细胞增殖核相关抗原Ki67指数,观察糖尿病大鼠骨折后成骨细胞的增殖能力。方法Wistar雄性大鼠40只,随机分为两组。实验组腹腔内注射链脲佐菌素破坏胰岛细胞诱导为糖尿病大鼠,对照组为正常组。两组大鼠均折断其右胫腓骨,然后复位外固定。于骨折后第1、2、4、6周拍x线片观察骨折愈合情况,取骨痂切片行HE染色光镜下观察,免疫组化测定Ki67指数。结果实验组第1、2、4、6周X线片上骨痂生成量骨痂内成骨细胞数量均明显少于对照组;Ki67指数第2周实验组为(18．06±3．54)％,对照组为(26．87±4．61)％,两组差异有统计学意义(P<0．01)。结论糖尿病大鼠骨折愈合过程中,骨痂中的成骨细胞增殖能力下降是导致糖尿病骨折大鼠愈合差的原因之一。
Objective To determine the Ki67 index of proliferating nuclear antigen (PCNA) in callus of diabetic rats after fracture and to observe the proliferation of osteoblasts after fracture in diabetic rats. Methods Forty male Wistar rats were randomly divided into two groups. Experimental group intraperitoneal injection of streptozotocin destroyed islet cells induced diabetic rats, the control group was normal group. Two groups of rats were broken off the right tibia and fibula, and then reset external fixation. At the first, second, fourth and sixth weeks after the fracture, the x-ray films were taken to observe the fracture healing. The callus slices were observed under light microscope and Ki67 index by immunohistochemistry. Results The number of callus in callus on the X-ray and the number of osteoblasts in the callus at 1, 2, 4 and 6 weeks in the experimental group were significantly less than those in the control group. The Ki67 index in the second week was (18.06 ± 3.54) %, While the control group was (26.87 ± 4.61)%, the difference between the two groups was statistically significant (P <0.01). Conclusions During the process of fracture healing in diabetic rats, the decrease of osteoblast proliferative capacity in callus is one of the causes of poor healing in diabetic rats.