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通过转录组测序获得了斑玉蕈(Hypsizygus marmoreus)SIEF3133菌株的β-葡萄糖苷酶基因的cDNA序列(命名为bgl1),采用荧光定量PCR检测bgl1的相对表达量。当PDB中加入0.05g/L的皂苷溶液,菌丝培养4~6d时,bgl1相对表达量降低了1/2左右(相比对照),8d开始升高,第10天为对照组的1.8倍;向工厂化生产用的栽培瓶(接种培养85d后搔菌处理)中添加0.05g/L的皂苷溶液15mL,可缩短子实体采收时间约3d(与对照组相比),在此过程中bgl1相对表达量在第5天时最低,20d时超过对照组,5d时为对照组的1.66倍。
The cDNA sequence of β-glucosidase gene (named as bgl1) of SIEF3133 strain of Hypsizygus marmoreus was obtained by transcriptome sequencing. The relative expression of bgl1 was detected by real-time PCR. When 0.05g / L saponin solution was added into PDB, the relative expression of bgl1 decreased about 1/2 after 4 ~ 6d mycelial culture (compared with the control), and began to increase at 8d, and was 1.8 times higher than the control group on the 10th day ; To the factory production of cultivation bottles (inoculation culture 85d after the scratch bacteria treatment) by adding 0.05g / L of 15mL saponin solution, can shorten the fruiting body harvest time of about 3d (compared with the control group), in the process The relative expression level of bgl1 was the lowest on the 5th day, more than the control group on the 20th day, and 1.66 times of the control group on the 5th day.