论文部分内容阅读
目的探讨中国汉族人白细胞介素10基因(interleukin10gene,IL10)启动子区单核苷酸多态性与乙型肝炎病毒(hepatitisBvirus,HBV)感染、转归的关联。方法采用聚合酶链反应-限制性片段长度多态性分析方法,检测231例HBV感染者,165例HBV感染康复者和135名正常对照者IL10基因启动子-1082G/A、-819T/C、-592A/C位点基因型。结果IL10基因启动子-1082G/A、-819T/C、-592A/C位点基因型和等位基因在HBV感染组、HBV感染康复组和正常对照组之间的分布频率比较差异无统计学意义(P>0.05),在血清HBV-DNA<1×103拷贝/mL的HBV感染者组和HBV-DNA≥1×103拷贝/mL组之间的分布频率比较差异亦无统计学意义(P>0.05);但IL10基因启动子-819T/C和-592A/C位点基因型和等位基因在HBV无症状携带组和慢性乙型肝炎组之间的分布差异有统计学意义(P<0.05),-819T/C位点TT型和-592A/C位点AA型在慢性乙型肝炎组的频率明显较高。结论汉族人IL10基因启动子多态性可能与人群对HBV易感性及感染后的病毒血症水平无显著相关性;但IL10启动子-819T/C和-592A/C位点基因多态性与HBV感染后的肝脏炎症反应有关。
Objective To investigate the association between single nucleotide polymorphisms in the promoter region of interleukin 10 gene (IL10) and the infection and prognosis of hepatitis B virus (HBV) in Chinese Han population. Methods The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to detect IL10 gene promoter-10182G / A and -819T / C in 231 patients with HBV infection, 165 patients with HBV infection and 135 healthy controls. -592A / C locus genotype. Results There were no significant differences in frequencies of genotypes and alleles of -1082G / A, -819T / C and -592A / C loci in IL10 gene between HBV infected group, HBV infected group and normal control group (P> 0.05). There was no significant difference in the frequencies of HBV-DNA between 1 × 103 copies / mL group and HBV-DNA≥1 × 103 copies / mL group (P > 0.05). However, the genotypes and alleles of -819T / C and -592A / C loci in IL10 gene were significantly different between HBV-infected group and chronic hepatitis B group (P < 0.05). The genotype TT of -819T / C site and AA genotype -592A / C were significantly higher in chronic hepatitis B patients. CONCLUSION: The polymorphism of IL10 gene promoter in Han population may not be related to the susceptibility to HBV and the level of viremia after infection. However, the polymorphisms of IL-10 -819T / C and -592A / C Liver inflammation after HBV infection related.