论文部分内容阅读
研究了温度、ｐＨ、酶量和起始乳糖浓度对低聚半乳糖合成的影响，发现乳糖水解为单糖和合成低聚糖所需的最佳条件并不相同；利用有机溶剂作反应介质有利于低聚糖合成。在疏水性环己烷／水（９５／５，Ｖ／Ｖ）反应体系中得到的低聚糖浓度为５２．６ｇ／Ｌ，占总产物的３９．１９％；在亲水性乙酸乙酯／水（８０／２０，Ｖ／Ｖ）体系中得到低聚糖的浓度为４０．０４ｇ／Ｌ，占总产物的３９．６２％。另外，疏水性有机溶剂抑制低聚糖水解的能力强于亲水性溶剂。
The effects of temperature, pH, amount of enzyme and initial lactose concentration on the synthesis of galactooligosaccharides were studied. The optimal conditions for the hydrolysis of lactose to monosaccharide and oligosaccharide were not the same. The organic solvent was used as reaction medium Conducive to oligosaccharides synthesis. The concentration of oligosaccharides in the hydrophobic cyclohexane / water (95/5, V / V) reaction system was 52.6 g / L, accounting for 39.19% of the total product. In the presence of hydrophilic ethyl acetate / The concentration of oligosaccharides in water (80/20, V / V) system was 40.04g / L, accounting for 39.62% of the total product. In addition, hydrophobic organic solvents inhibit the hydrolysis of oligosaccharides stronger than hydrophilic solvents.