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目的探讨宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)及宫颈癌组织中EZH2与VEGF-C的表达情况、临床意义及其相关性。方法选取65例宫颈癌石蜡标本及90例CIN标本(其中CINⅠ、CINⅡ、CINⅢ各30例)为观察组,同时随机选取30例正常宫颈组织为对照组。应用免疫组化SP法对宫颈癌、CIN组织和正常宫颈组织中的EZH2与VEGF-C的表达进行测定。结果观察组EZH2、VEGF-C的表达阳性率高于对照组,差异有统计学意义;三者组间比较差异有统计学意义(P<0.05)。EZH2与VEGF-C的阳性表达均与CIN组织病理学分级显著相关,而与宫颈癌临床分期无显著相关性。相关性分析显示,EZH2与VEGF-C在宫颈癌中的表达呈正相关(P<0.05)。结论 EZH2与VEGF-C的异常表达与宫颈癌发生和进展密切相关,其机制可能与二者协同促进肿瘤脉管生成有关。
Objective To investigate the expression, clinical significance and correlation of EZH2 and VEGF-C in cervical intraepithelial neoplasia (CIN) and cervical cancer. Methods 65 cases of cervical cancer paraffin specimens and 90 cases of CIN specimens (including CIN Ⅰ, CIN Ⅱ, CIN Ⅲ 30 cases) were selected as the observation group, while 30 cases of normal cervical tissue were randomly selected as the control group. Immunohistochemical SP method was used to detect the expression of EZH2 and VEGF-C in cervical cancer, CIN tissues and normal cervical tissues. Results The positive expression rate of EZH2 and VEGF-C in the observation group was higher than that in the control group, with statistical significance (P <0.05). The positive expression of EZH2 and VEGF-C were significantly correlated with the histological grade of CIN, but not with the clinical stage of cervical cancer. Correlation analysis showed that the expression of EZH2 and VEGF-C in cervical cancer was positively correlated (P <0.05). Conclusion The abnormal expression of EZH2 and VEGF-C is closely related to the occurrence and progression of cervical cancer, which may be related to their synergistic effect on tumor angiogenesis.