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目的研究8-烷基小檗碱的抗焦虑作用,初步探讨其作用机制。方法通过明暗箱和高架十字迷宫实验,观察8-烷基小檗碱对焦虑模型小鼠行为学的影响;采用高效液相-荧光检测器,检测小鼠脑组织内神经递质去甲肾上腺素(NE)、多巴胺(DA)和5-羟色胺(5-HT)的量。结果明暗箱实验结果表明,相对于对照组,8-己基小檗碱、8-辛基小檗碱、8-十二烷基小檗碱和8-十六烷基小檗碱给药后能显著延长小鼠在明箱内的停留时间(P<0.05),8-辛基小檗碱组的穿箱次数显著增多(P<0.05);高架十字迷宫实验结果显示,8-辛基小檗碱组小鼠进入开臂次数百分比和开臂滞留时间百分比明显高于对照组(P<0.05);小鼠脑内神经递质NE、DA和5-HT的量变化以8-辛基小檗碱组最为明显。结论 8-烷基小檗碱具有一定的抗焦虑作用,其作用机制可能与降低脑组织中NE、DA和5-HT的量有关。
Aim To study the anti-anxiety effect of 8-alkyl-berberine and its mechanism of action. Methods The effects of 8-alkyl-berberine on anxiety model mice were observed by light and dark box and elevated plus maze test. The neurotransmitter norepinephrine (NE), dopamine (DA) and serotonin (5-HT). Results The results of the light and dark box experiments showed that compared with the control group, 8-hexylberberine, 8-octylberberine, 8-dodecylberberine and 8-hexadecylberberine (P <0.05), and the number of wearing boxes in 8-octylberberine group increased significantly (P <0.05). The results of elevated plus-maze test showed that 8-Octylberberry (P <0.05). The amount of neurotransmitters NE, DA and 5-HT in the brain of mice in the alkali group was significantly higher than that in the control group (P <0.05) Alkali group is the most obvious. Conclusion 8-alkylberberine has some anti-anxiety effects, and its mechanism may be related to the decrease of NE, DA and 5-HT in brain tissue.