利用可控组装方法制备了单仓脂质体.这种制备方法分为两个步骤;(a)利用超声振荡形成油包水(W/O)的微乳液,(b)微乳中小水滴在离心作用下通过油水界面的单分子膜并被其包裹,在水相中形成脂质体.脂质体直径在50～200nm.脂质体的双分子膜间基本没有残留有机溶剂存在,并且在制备过程中包封的试剂没有泄漏.可控组装方法制备脂质体具有操作简单、生成脂质体一般不需进一步分离、包封效率较高(57%)等特点. A single chamber liposome was prepared by a controlled assembly method, which was divided into two steps: (a) ultrasonic emulsification to form a water-in-oil (W / O) microemulsion, (b) Centrifugal action through the oil-water interface of the monolayer and was wrapped in the aqueous phase to form liposomes in the liposome diameter of 50 ~ 200nm liposome bimetallic membranes were basically no residual organic solvents exist, and in the There is no leakage of the encapsulated reagent in the preparation process Controlled assembly method The preparation of liposomes has the advantages of simple operation, generation of liposomes generally without further separation, and higher encapsulation efficiency (57%).