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目的：探讨内质网应激因子TRB3在不同月龄APP／PS1转基因小鼠脑内的表达情况，阐明TRB3与阿尔茨海默病（AD）病情进展的关系。方法将APP／PS1转基因小鼠分为3月龄WT组、3月龄APP／PS1组、7月龄WT组、7月龄APP／PS 1组，每组各10只。分别以3月龄、7月龄野生型（WT ）C57／B L6 J小鼠为对照组，每组10 只。采用Morris水迷宫实验检测小鼠行为学改变，采用免疫组织化学及Western blotting方法检测TRB3在小鼠脑内的表达。结果与同月龄WT组比较，3月龄APP／PS1组未出现明显行为学改变（P＞0．05），而7月龄APP／PS1组学习记忆能力下降，差异有统计学意义（P＜0．05）；TRB3主要在大脑皮层表达，与同月龄WT组比较，TRB3在3月龄及7月龄APP／PS1组脑内的表达均明显增多（P＜0．05），且7月龄APP／PS1组脑内TRB3的表达水平明显高于3月龄APP／PS1组（P＜0．05）。结论 TRB3在APP／PS1转基因小鼠脑内表达增多，且TRB3表达水平的增多可能与AD的病情进展有关。“,”Objective To explore the expression of endoplasmic reticulum stress factor TRB3 in the brain of APP/PS1 transgenic mice of different months,and to clarify its relation with the progression of Alzheimers disease.Methods APP/PS1 transgenic mice in 3 and 7 months old were used in this study and wild type (WT)C57/BL6J mice were used as control group.Mice were divided into 3-month old WT group,3-month old APP/PS1 group,7-month old WT group and 7-month old APP/PS1 group,and each group had 10 mice.Behavioral changes of mice were determined by Morris Water Maze,and the expression of TRB3 in the brain was studied by immunohistochemistry and Western blot-ting.Results Compared with WT mice of same months,there were no significant behavioral test changes in 3-month old APP/PS1 group (P>0.05),and the decline of learning and memory ability was found in 7-month old APP/PS1 group,which showed statistical difference compared with WT mice of same months (P<0.05 ).TRB3 was mainly expressed in brain cortex.Compared with WT mice of same months,TRB3 expression increased significantly in the brain of both 3 and 7-month old APP/PS1 mice (P<0.05),and the expression level of TRB3 in the brain of 7-month old APP/PS1 group was significantly higher than that of 3-month old APP/PS1 group(P<0.05).Conclusion TRB3 shows higher expression level in the brain of APP/PS1 transgenic mice and its increase may be related to the progression of Alzheimers disease.