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用3．5L和14L生物反应器培养WUT3杂交瘤细胞，结果表明用人血清可以代替牛血清培养WuT3细胞，在1％人血清培养液中添加适当营养成分，可达到10％小牛血清浓度下的细胞浓度（1．5×106／ml）和单抗产量（大于50μg／ml）。放大到75L生物反应器中采用半连续方式培养，建立了中试培养工艺流程和质控点，收获时细胞浓度为1.0×106～1．5×106／ml，单抗产量为40～70μg／ml，平均日产单抗达1g。在3．5L、14L、75L逐级放大培养过程中，细胞支原体为阴性，收获上清中单抗免疫活性合格，热原试验合格。
The WUT3 hybridoma cells were cultured in 3.5L and 14L bioreactor. The results showed that WuT3 cells could be cultured in human serum instead of bovine serum, and the appropriate nutrient content in 1% human serum culture medium could reach 10% Cell concentration (1.5 × 10 6 / ml) and mAb production (greater than 50 μg / ml). Amplified to 75L bioreactor using semi-continuous culture, the establishment of the pilot culture process and quality control points, the cell density at harvest 1.0 × 106 ~ 1.5 × 106 / ml, monoclonal antibody production was 40 ~ 70μg / ml, the average daily production of monoclonal antibody up to 1g. In 3.5L, 14L, 75L gradual amplification process, the cell mycoplasma was negative, the harvested monoclonal antibody immunocompetence in the supernatant, pyrogen test pass.