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目的:探讨生物波调控因子(BRF)对实验性脑梗死大鼠脑组织NF-κB表达的影响。方法:成年健康雄性SD大鼠90只随机假手术组、生理盐水组、BRF治疗组。制备大脑中动脉梗死(MCAO)模型,术后1h以1ml/100g的剂量分别腹腔注射1.25%BRF溶液和生理盐水,此后1次/d。进行行为学评分、干湿重法测定脑组织含水量、HE染色观察组织病理学改变、免疫组织化学方法测定脑组织NF-κB的表达。结果:(1)MCAO模型大鼠术后各时间点BRF治疗组的行为学评分较生理盐水组降低。(2)与假手术组比较,BRF治疗组和生理盐水组缺血脑组织含水量于24h开始明显升高,48h达高峰,持续至72h,7d明显下降。除6h外在各时间点均有显著性差异。BRF治疗组脑组织水肿程度减轻,48h脑含水量明显低于生理盐水组。(3)缺血区炎细胞浸润和NF-κB阳性细胞表达于梗死后6h开始增多,48h达高峰,持续至7d。假手术组未见明显炎细胞浸润,可见少量散在的NF-κB阳性细胞。BRF治疗组病理损伤减轻,术后48h、72h脑组织NF-κB阳性细胞表达明显低于生理盐水组。结论:生物波调控因子可以减轻梗死后脑水肿,降低脑组织NF-κB的表达,对大鼠脑组织的缺血损伤产生保护作用。
Objective: To investigate the effect of bio-wave factor (BRF) on the expression of NF-κB in rat brain after experimental cerebral infarction. Methods: Ninety adult male Sprague-Dawley rats were randomly divided into sham operation group, saline group and BRF treatment group. Middle cerebral artery occlusion (MCAO) model was prepared. The rats were injected intraperitoneally with 1.25% BRF solution and normal saline at a dose of 1 ml / 100 g 1 h after operation and once a day thereafter. The behavioral score was scored. The water content of brain tissue was determined by wet and dry method. The histopathological changes were observed by HE staining. The expression of NF-κB in brain tissue was determined by immunohistochemical method. Results: (1) The behavioral score of BRF treatment group was lower than that of saline control group at each time point after MCAO. (2) Compared with the sham-operation group, the water content of ischemic brain tissue in BRF-treated group and saline-treated group increased significantly at 24h, peaked at 48h, and continued to 72h and decreased significantly at 7d. In addition to 6h at all time points were significantly different. Brain edema in BRF treatment group was alleviated, and the brain water content in 48h was significantly lower than that in saline group. (3) Inflammatory cell infiltration and expression of NF-κB positive cells in ischemic area began to increase at 6h after infarction, reached the peak at 48h and lasted for 7 days. Sham-operated group showed no obvious infiltration of inflammatory cells, showing a small amount of scattered NF-κB positive cells. BRF treatment group, pathological damage alleviated, 48h, 72h after the expression of NF-κB positive cells was significantly lower than the saline group. Conclusion: The biological wave regulators can reduce brain edema after infarction, reduce the expression of NF-κB in brain tissue, and protect the cerebral ischemia-reperfusion injury in rats.