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以抗坏血酸为阳性对照,分别对桑木层孔菌Phellinus mori、鲍姆木层孔菌Phellinus baumii和瓦宁木层孔菌Phellinus vaninii野生子实体甲醇提取物(ME)、氯仿提取物(CE)、乙酸乙酯提取物(EAE)、正丁醇提取物(BE)和热水提取物(WE)的体外清除DPPH(1,1-二苯基-2-三硝基苯肼)自由基、羟自由基和总抗氧化能力进行了检测。结果表明,3种木层孔菌的不同溶剂提取物都具有体外抗氧化活性。3种菌EAE清除DPPH自由基能力较强,其中瓦宁木层孔菌EAE清除DPPH自由基能力最强,IC50为10.65μg/mL。3种木层孔菌热水浸提物清除羟自由基的能力较强,鲍姆木层孔菌WE的清除率最高,1mg/mL时达到73.52%。另外,3种木层孔菌不同提取物总抗氧化能力和清除DPPH自由基能力基本相同,瓦宁木层孔菌BE的总抗氧化能力最强,1mg/mL时达到了167.7U/mL。3种木层孔菌相比,瓦宁木层孔菌不同提取物的体外抗氧化能力最强,其次是鲍姆木层孔菌,桑木层孔菌的体外抗氧化能力最弱。
Ascorbic acid was used as a positive control, respectively, for Phellinus mori, Phellinus baumii and Phellinus vaninii wild fruiting body methanol extract (ME), chloroform extract (CE), acetic acid DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) free radical scavenging of ethyl ester extract (EAE), n-butanol extract (BE) and hot water extract (WE) Base and total antioxidant capacity were tested. The results showed that the three kinds of Pseudomonas aeruginosa different solvent extracts have in vitro antioxidant activity. The ability of the three kinds of bacteria to scavenge DPPH radical was stronger. The ability of EAE to eliminate DPPH radical was the strongest, with IC50 of 10.65μg / mL. The three kinds of Mycopsis pleuropneumoniae extract of the hydroxyl radical scavenging capacity of the stronger, the highest clearance rate of Ulmus pumilus WE, 1mg / mL reached 73.52%. In addition, the total antioxidant capacity and DPPH free radical scavenging capacity of different extracts of the three kinds of Pseudomonas aeruginosa were basically the same. The total antioxidant capacity of Pseudomonas wallichii BE was the strongest, reaching 167.7U / mL at 1mg / mL. Compared with the three kinds of Pseudomonas aeruginosa, the different extracts of Phellinus igniarius had the strongest antioxidant activity in vitro, followed by the Pseudomonas aeruginosa and the Pseudomonas parva.