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通过电化学聚合在钛基材表面构建聚多巴胺膜层,探讨了pH诱导聚多巴胺膜表面质子化/去质子化及其电势电荷的变化特性,并研究了其对骨髓间充质干细胞(BMSCs)的早期黏附行为的影响.利用拉曼光谱和场发射扫描电镜证实,通过电化学方法在钛表面可构建均匀致密的聚合多巴胺膜层;电化学及动力学分析表明,不同pH介质条件下,聚多巴胺膜的界面电阻不同,且其随着pH的增大而增大,质子化的聚多巴胺有利于电荷累积;原子力显微镜结果表明,质子化和去质子化的聚多巴胺膜表面电势,在不同p H环境下具有可逆周期性变化的特性;探讨了膜层质子化作用对BMSCs的黏附作用机理,表明质子化的聚多巴胺钛表面带正电荷更有利于细胞的黏附和铺展,且具有良好的细胞相容性.
The polydopamine film was formed on the surface of titanium substrate by electrochemical polymerization. The protonation / deprotonation of the surface of pH-induced polydopamine film and the change of its potential charge were also studied. The effects of BMSCs on BMSCs, The results of Raman spectroscopy and field emission scanning electron microscopy showed that the uniform and dense polymeric dopamine film could be formed on the surface of titanium by electrochemical method. Electrochemical and kinetic analysis showed that under different pH conditions, poly The interfacial resistance of dopamine film is different, and it increases with the increase of pH. The protonated polydopamine is conducive to the charge accumulation. The results of AFM show that the surface potentials of protonated and deprotonated polydopamine films vary with different p H has the characteristics of reversible cyclical changes; discussed the protonation of the membrane BMSCs adhesion mechanism, indicating that protonated poly dopamine titanium surface is more conducive to the positive cell surface adhesion and spreading, and has good cells compatibility.